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Green Synthesis of Gold Nanoparticles Coupled with Nucleic Acid Oxidation
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2017-11-17 00:00:00 , DOI: 10.1021/acssuschemeng.7b02610
Tatsuki Kunoh , Minoru Takeda 1 , Syuji Matsumoto , Ichiro Suzuki 1 , Mikio Takano , Hitoshi Kunoh , Jun Takada
Affiliation  

Green synthesis of metal nanoparticles, especially gold nanoparticles (AuNPs), has attracted the great interest of scientists and engineers in the medical and pharmaceutical fields; thus, a variety of ecofriendly, energy- and cost-saving techniques have been developed. In this study, we found that cells of Leptothrix (iron-oxidizing bacteria) released extracellular RNA some of which could exist as a constituent of the cell-enclosing sheaths. As a part of studies of metal encrustation in the sheaths, here we show that RNA prepared from the Leptothrix cells can reduce Au(III) and spherical AuNPs eventually form when an aqueous HAuCl4 solution is added under ambient conditions. RNA and DNA of other organismal origins have the same ability. Of the nucleosides and nucleobases, only guanosine and guanine can form AuNPs. The DNA moiety, 2′-deoxyguanosine (dG) (used as a reference material), forms AuNPs when mixed with HAuCl4 solution, but 8-hydroxy-2′-deoxyguanosine (8-OHdG) does not, indicating that AuNP formation evidently depends on the reduction potential of the guanine moiety, not the sugar moiety. This finding is the first demonstration that spherical AuNPs of ca. 5 nm diameter can be obtained by simply adding guanine to HAuCl4 solution at ambient temperature; no other chemicals or physical treatments are needed.

中文翻译:

金纳米粒子的绿色合成及其与核酸的氧化作用

金属纳米粒子,特别是金纳米粒子(AuNPs)的绿色合成,已经引起了医学和制药领域科学家和工程师的极大兴趣。因此,已经开发了多种环保,节能和节省成本的技术。在这项研究中,我们发现Leptothrix(铁氧化细菌)的细胞释放了细胞外RNA,其中一些可能作为细胞封闭鞘的成分存在。作为研究鞘中金属结壳的一部分,我们在这里表明,从Leptothrix细胞制备的RNA可以还原Au(III),而球形HANPl 4最终形成球形AuNPs。在环境条件下添加溶液。其他生物来源的RNA和DNA具有相同的功能。在核苷和核碱基中,只有鸟苷和鸟嘌呤可以形成AuNPs。当与HAuCl 4溶液混合时,DNA部分2'-脱氧鸟苷(dG)(用作参考材料)形成AuNP ,而8-羟基-2'-脱氧鸟苷(8-OHdG)则不形成AuNP,这表明明显形成了AuNP。取决于鸟嘌呤部分而不是糖部分的还原电位。这一发现是第一个证明球形金纳米粒子约。只需在环境温度下将鸟嘌呤添加到HAuCl 4溶液中即可获得5 nm的直径;无需其他化学或物理处理。
更新日期:2017-11-19
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