Silica nanoparticles based on an AIE-active molecule for ratiometric detection of RNS in vitro,Journal of Materials Chemistry B

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Silica nanoparticles based on an AIE-active molecule for ratiometric detection of RNS in vitro
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-11-06 00:00:00 , DOI: 10.1039/c7tb02734f
Leijing Liu _{1,

2,

3} , Fengli Zhang _{1,

2,

3} , Bin Xu _{1,

2,

3} , Wenjing Tian _{1,

2,

3}

Affiliation

Nitric oxide (NO), known as a reactive nitrogen species (RNS), has been considered to be a significant factor in many cell-related biological processes. There is a great desire to develop fluorescent probes that can highly sensitively and selectively detect NO in living cells. Herein, a fluorescent probe, a tetraphenylethene (TPE) derivative TPE-2NH2, with aggregation induced emission (AIE) properties was designed and synthesized. Then silica nanoparticles based on TPE-2NH2 were fabricated for ratiometric detection of NO in vitro with high sensitivity and selectivity. In the presence of NO, the fluorescence of the silica nanoparticles changes from 519 nm (green) to 655 nm (red) because of the chemical reaction between NO and o-phenylenediamine in TPE-2NH2. The silica nanoparticles exhibited very high sensitivity and selectivity towards NO in aqueous buffer, and the response time was as short as 5 minutes. Moreover, the silica nanoparticles can also highly sensitively and selectively respond to NO produced in living cells. When MCF-7 cancer cells were activated by lipopolysaccharides (LPS), which can effectively induce the production of NO, the red emission signal of the silica nanoparticles obviously increased. This demonstrated that the silica nanoparticles showed fine imaging contrast towards NO in MCF-7 cancer cells, which provides a facile tool to detect intracellular NO.

中文翻译：

二氧化硅纳米粒子基于用于检测比例RNS的AIE活性分子在体外

一氧化氮（NO），称为反应性氮物质（RNS），已被认为是许多与细胞有关的生物过程中的重要因素。迫切需要开发能够高度灵敏并选择性地检测活细胞中NO的荧光探针。本文中，设计并合成了具有聚集诱导发射（AIE）特性的荧光探针四苯乙烯（TPE）衍生物TPE-2NH2。然后制备了基于TPE-2NH2的二氧化硅纳米粒子，用于体外比例测定NO的灵敏度和选择性高。在NO的存在下，由于NO和o之间的化学反应，二氧化硅纳米粒子的荧光从519 nm（绿色）变为655 nm（红色）。-TPE-2NH2中的-苯二胺。二氧化硅纳米颗粒在水性缓冲液中表现出非常高的灵敏度和对NO的选择性，响应时间短至5分钟。此外，二氧化硅纳米颗粒还可以高度敏感地和选择性地响应活细胞中产生的NO。当脂多糖（LPS）激活MCF-7癌细胞可有效诱导NO生成时，二氧化硅纳米粒子的红色发射信号明显增加。这表明二氧化硅纳米颗粒在MCF-7癌细胞中显示出与NO良好的成像对比，这提供了一种检测细胞内NO的简便工具。

更新日期：2017-11-17

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