MethBank (http://bigd.big.ac.cn/methbank) is a database that integrates high-quality DNA methylomes across a variety of species and provides an interactive browser for visualization of methylation data. Here, we present an updated implementation of MethBank (version 3.0) by incorporating more DNA methylomes from multiple species and equipping with more enhanced functionalities for data annotation and more friendly web interfaces for data presentation, search and visualization. MethBank 3.0 features large-scale integration of high-quality methylomes, involving 34 consensus reference methylomes derived from a large number of human samples, 336 single-base resolution methylomes from different developmental stages and/or tissues of five plants, and 18 single-base resolution methylomes from gametes and early embryos at multiple stages of two animals. Additionally, it is enhanced by improving the functionalities for data annotation, which accordingly enables systematic identification of methylation sites closely associated with age, sites with constant methylation levels across different ages, differentially methylated promoters, age-specific differentially methylated cytosines/regions, and methylated CpG islands. Moreover, MethBank provides tools to estimate human methylation age online and to identify differentially methylated promoters, respectively. Taken together, MethBank is upgraded with significant improvements and advances over the previous version, which is of great help for deciphering DNA methylation regulatory mechanisms for epigenetic studies.

中文翻译：

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MethBank 3.0：各种物种的DNA甲基化组数据库

MethBank（http://bigd.big.ac.cn/methbank）是一个数据库，该数据库整合了各种物种的高质量DNA甲基化组，并提供了用于可视化甲基化数据的交互式浏览器。在这里，我们通过结合来自多个物种的更多DNA甲基基因组，并配备了用于数据注释的增强功能和用于数据表示，搜索和可视化的更友好的Web界面，提供了MethBank（3.0版）的更新实现。MethBank 3.0具有高质量甲基化组的大规模整合功能，涉及来自大量人类样品的34个共有参考甲基化组，五种植物不同发育阶段和/或组织的336个单碱基分辨率的甲基化组。分辨来自两只动物多个阶段的配子和早期胚胎的甲基化组。此外，它通过改善数据注释功能而得到增强，从而可以系统地识别与年龄密切相关的甲基化位点，跨不同年龄具有恒定甲基化水平的位点，差异甲基化的启动子，特定年龄差异的甲基化胞嘧啶/区域和甲基化的CpG岛。 。此外，MethBank提供了在线评估人类甲基化年龄并分别识别差异甲基化启动子的工具。总之，MethBank进行了升级，与以前的版本相比有了显着的改进和进步，这对于破译表观遗传学研究的DNA甲基化调节机制有很大帮助。各个年龄段具有恒定甲基化水平的位点，差异甲基化启动子，特定年龄差异甲基化胞嘧啶/区域和甲基化CpG岛。此外，MethBank提供了在线评估人类甲基化年龄并分别识别差异甲基化启动子的工具。总之，MethBank进行了升级，与以前的版本相比有了显着的改进和进步，这对于破译表观遗传学研究的DNA甲基化调节机制有很大帮助。各个年龄段具有恒定甲基化水平的位点，差异甲基化启动子，特定年龄差异甲基化胞嘧啶/区域和甲基化CpG岛。此外，MethBank提供了在线评估人类甲基化年龄并分别识别差异甲基化启动子的工具。总之，MethBank进行了升级，与以前的版本相比有了显着的改进和进步，这对于破译表观遗传学研究的DNA甲基化调节机制有很大帮助。