A novel and sensitive strategy for procalcitonin (PCT) determination was developed based on zinc nanoparticles-functionalized ordered mesoporous carbon-silica nano-composites (OMCSi-Zn). This strategy combined an effectively designed trace tag and a simple immunosensor. The large amount of OMCSi-Zn materials as label greatly amplified the detection signals for the electrochemical detection of proteins, while the good biocompatibility of graphene-chitosan as sensor matrix retained excellent stability and accelerated electron transfer for the sandwich-type immunoassay. Without metal preconcentration, the zinc nanoparticles loading on the ordered mesoporous carbon-silica nano-composites (OMCSi) could directly be detected through electrochemical voltammetries, while the immunoreaction had a close relationship with the distinct voltammetric peaks. The proposed method exhibited high sensitivity and good stability for PCT detection, possessing an improved linear response range from 0.05 pg/mL to 80 ng/mL, with a detection limit of 0.013 pg/mL (S/N = 3). Moreover, the serum samples from clinic were accurately detected with the proposed method, indicating that the developed immunoassay possessed excellent potential application in clinical diagnostics for PCT.

基于锌纳米粒子功能化的有序介孔碳-二氧化硅纳米复合材料（OMCSi-Zn），开发了一种新的敏感的降钙素原（PCT）测定策略。该策略结合了有效设计的跟踪标签和简单的免疫传感器。大量的OMCSi-Zn材料作为标记物极大地放大了蛋白质电化学检测的检测信号，而石墨烯-壳聚糖作为传感器基质的良好生物相容性则为三明治型免疫测定法保留了出色的稳定性和加速的电子转移。如果不进行金属预富集，则可以通过电化学伏安法直接检测到有序介孔碳-二氧化硅纳米复合材料（OMCSi）上负载的锌纳米颗粒，而免疫反应则与不同的伏安峰密切相关。所提出的方法对PCT检测具有较高的灵敏度和稳定性，具有从0.05 pg / mL到80 ng / mL的改进的线性响应范围，检测限为0.013 pg / mL（S / N = 3）。此外，用所提出的方法准确地检测了来自临床的血清样品，表明所开发的免疫测定法在PCT的临床诊断中具有极好的潜在应用价值。