Surfactant‐Free β‐Galactosidase Micromotors for “On‐The‐Move” Lactose Hydrolysis,Advanced Functional Materials

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Surfactant‐Free β‐Galactosidase Micromotors for “On‐The‐Move” Lactose Hydrolysis
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2017-11-17 , DOI: 10.1002/adfm.201704256
Roberto Maria-Hormigos ₁ , Beatriz Jurado-Sánchez _{1,

2} , Alberto Escarpa _{1,

2}

Affiliation

Surfactant‐free β‐galactosidase micromotors are explored here as moving biocatalyst for highly efficient lactose hydrolysis from raw milk. The coupling of the hydrolytic properties of such enzyme with the efficient movement of carbon nanotube tubular micromotors results in nearly 100% lactose hydrolysis and two fold removal efficiency as compared with static conditions and with free enzyme. The incorporation of an inner Ni layer allows its reusability to operate in batch mode. The rough micromotor surface area allows the immobilization of a high loading of β‐galactosidase and results in an increase in the enzyme affinity toward lactose. The new micromotor concept opens new avenues for the use of micromotors as moving immobilized biocatalyst to improve the technological process not only in food industry but also in other fields.

中文翻译：

不含表面活性剂的β-半乳糖苷酶微电机，可“移动”乳糖水解

不含表面活性剂的β-半乳糖苷酶微电机在这里被用作移动生物催化剂，用于从生乳中高效水解乳糖。与静态条件和游离酶相比，这种酶的水解特性与碳纳米管管状微型马达的有效运动的结合导致近100％的乳糖水解和两倍的去除效率。内层镍的引入允许其可重复使用性以批处理模式运行。粗糙的微电机表面积可固定高负荷的β-半乳糖苷酶，并导致酶对乳糖的亲和力增加。新的微电机概念为使用微电机作为移动的固定化生物催化剂开辟了新途径，不仅改善了食品工业的技术工艺，而且还改善了其他领域的技术工艺。

更新日期：2017-11-17

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