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Aptamer-Templated Silver Nanoclusters Embedded in Zirconium Metal–Organic Framework for Bifunctional Electrochemical and SPR Aptasensors toward Carcinoembryonic Antigen
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2017-11-16 00:00:00 , DOI: 10.1021/acsami.7b14952 Chuanpan Guo 1 , Fangfang Su 1 , Yingpan Song 1 , Bin Hu 1 , Minghua Wang 1 , Linghao He 1 , Donglai Peng 1 , Zhihong Zhang 1
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2017-11-16 00:00:00 , DOI: 10.1021/acsami.7b14952 Chuanpan Guo 1 , Fangfang Su 1 , Yingpan Song 1 , Bin Hu 1 , Minghua Wang 1 , Linghao He 1 , Donglai Peng 1 , Zhihong Zhang 1
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This study reported a novel biosensor based on the nanocomposite of zirconium metal–organic framework (Zr-MOF, UiO-66) embedded with silver nanoclusters (Ag NCs) using the carcinoembryonic antigen (CEA)-targeted aptamer as template (AgNCs@Apt@UiO-66). The synthesized AgNCs@Apt@UiO-66 nanocomposite not only possesses good biocompatibility, active electrochemical performance, and strong bioaffinity, but also can be dispersed to form two-dimensional nanocomposite with nanoscale thickness. As such, the use of the AgNCs@CEA-aptamer enables AgNC@Apt@UiO-66 with sensitive and selective detection capacity of trace CEA, further concurrently being exploited as scaffold for surface plasmon resonance spectroscopy (SPR) and electrochemical biosensors. The results showed that the proposed electrochemical AgNC@Apt@UiO-66-based aptasensor exhibits high sensitivity with a low detection limit (LOD) of 8.88 and 4.93 pg·mL–1 deduced from electrochemical impedance spectroscopy and differential pulse voltammetry, respectively, within a broad linear range of the CEA concentration (0.01–10 ng·mL–1). Meanwhile, the developed SPR biosensor exhibited a slightly high LOD of 0.3 ng·mL–1 within the CEA concentration of 1.0–250 ng·mL–1. Both the electrochemical and SPR aptasensors displayed high selectivity, good reproducibility, stability, acceptable regenerability, and applicability in real human serum samples. These results proved that the proposed aptamer-targeted Zr-MOF nanocomposite can be utilized in multiple-functionally biosensing, further promoting the potential application of Zr-MOF-related nanomaterials in clinical diagnosis.
中文翻译：
嵌入锆金属有机骨架中的适体模板银纳米簇，用于双功能电化学和SPR适应性传感器，用于癌胚抗原。
这项研究报告了一种新型的生物传感器，该传感器基于以锆胚抗原（CEA）为靶标的适体作为模板（AgNCs @ Apt @ UiO-66）。合成的AgNCs @ Apt @ UiO-66纳米复合材料不仅具有良好的生物相容性，电化学活性，生物亲和力强，而且可以分散形成具有纳米级厚度的二维纳米复合材料。因此，使用AgNCs @ CEA-aptamer可使AgNC @ Apt @ UiO-66具有痕量CEA的灵敏和选择性检测能力，并同时被用作表面等离子体共振光谱（SPR）和电化学生物传感器的支架。–1分别从CEA浓度的宽线性范围（0.01–10 ng·mL –1）中由电化学阻抗谱法和差分脉冲伏安法推导得出。同时，发达SPR生物传感器表现出0.3纳克的稍微高LOD·ML -1 1.0-250纳克的CEA浓度内·ML -1。电化学和SPR适体传感器均显示出高选择性，良好的重现性，稳定性，可接受的可再生性以及在实际人血清样品中的适用性。这些结果证明，所提出的适体靶向Zr-MOF纳米复合材料可用于多功能生物传感，进一步促进了Zr-MOF相关纳米材料在临床诊断中的潜在应用。
更新日期：2017-11-17
中文翻译：
嵌入锆金属有机骨架中的适体模板银纳米簇，用于双功能电化学和SPR适应性传感器，用于癌胚抗原。
这项研究报告了一种新型的生物传感器，该传感器基于以锆胚抗原（CEA）为靶标的适体作为模板（AgNCs @ Apt @ UiO-66）。合成的AgNCs @ Apt @ UiO-66纳米复合材料不仅具有良好的生物相容性，电化学活性，生物亲和力强，而且可以分散形成具有纳米级厚度的二维纳米复合材料。因此，使用AgNCs @ CEA-aptamer可使AgNC @ Apt @ UiO-66具有痕量CEA的灵敏和选择性检测能力，并同时被用作表面等离子体共振光谱（SPR）和电化学生物传感器的支架。–1分别从CEA浓度的宽线性范围（0.01–10 ng·mL –1）中由电化学阻抗谱法和差分脉冲伏安法推导得出。同时，发达SPR生物传感器表现出0.3纳克的稍微高LOD·ML -1 1.0-250纳克的CEA浓度内·ML -1。电化学和SPR适体传感器均显示出高选择性，良好的重现性，稳定性，可接受的可再生性以及在实际人血清样品中的适用性。这些结果证明，所提出的适体靶向Zr-MOF纳米复合材料可用于多功能生物传感，进一步促进了Zr-MOF相关纳米材料在临床诊断中的潜在应用。
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