Biomimetic Hyaluronic Acid-Lysozyme Composite Coating on AZ31 Mg Alloy with Combined Antibacterial and Osteoinductive Activities,ACS Biomaterials Science & Engineering

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Biomimetic Hyaluronic Acid-Lysozyme Composite Coating on AZ31 Mg Alloy with Combined Antibacterial and Osteoinductive Activities
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2017-11-16 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00527
Sankalp Agarwal _{1,

2} , Mathieu Riffault ₃ , David Hoey ₃ , Brendan Duffy ₁ , James Curtin ₂ , Swarna Jaiswal ₁

Affiliation

This study presents the covalent grafting of a hyaluronic acid-lysozyme (HA-LZ) composite onto corrosion-resistant silane-coated AZ31 Mg alloy via EDC-NHS coupling reactions. The HA-LZ composite coatings created a smooth and hydrophilic surface with the increased concentration of functional lysozyme complexed to the hyaluronic acid. This was confirmed by the measurement of AFM, water contact angle, and quantification of hyaluronic acid and lysozyme. The colonization of S.aureus on HA-LZ composite-coated substrates was significantly reduced as compared to the hyaluronic acid, lysozyme coated and uncoated AZ31 controls. Such activity is due to the enhanced antibacterial activity of lysozyme component as observed from the spread plate assay, propidium iodide staining, and scanning electron microscopy. Furthermore, morphology of the osteoblast cells, alkaline phosphatase activity and DNA quantification studies demonstrated the improved biocompatibility and osteoinductive properties of HA-LZ-coated substrates. This was verified by comparing with the lysozyme coated and uncoated AZ31 substrates in terms of cell adhesion, proliferation, and differentiation of osteoblastic cells. Therefore, such multifunctional composite coatings with antibacterial and osteoinductive properties are promising can be potentially used for the surface modifications of orthopedic implants.

中文翻译：

具有抗菌和骨诱导活性的AZ31镁合金仿生透明质酸-溶菌酶复合涂层

这项研究提出了透明质酸-溶菌酶（HA-LZ）复合材料通过EDC-NHS偶联反应共价接枝到耐腐蚀的硅烷包覆的AZ31 Mg合金上。HA-LZ复合涂层产生光滑且亲水的表面，并增加了与透明质酸复合的功能性溶菌酶的浓度。通过AFM的测量，水接触角以及透明质酸和溶菌酶的定量证实了这一点。金黄色葡萄球菌的定殖与透明质酸，溶菌酶包被的和未包被的AZ31对照相比，HA-LZ复合材料包被的底物的表面活性显着降低。这种活性归因于从铺板分析，碘化丙啶染色和扫描电子显微镜观察到的溶菌酶成分的增强的抗菌活性。此外，成骨细胞的形态，碱性磷酸酶活性和DNA定量研究表明，HA-LZ涂层基质的生物相容性和骨诱导性能得到改善。通过与溶菌酶包被和未包被的AZ31底物进行比较，在成骨细胞的细胞粘附，增殖和分化方面进行了验证。所以，

更新日期：2017-11-17

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