In this study, we developed a multifunctional chromatographic medium by using iminodisuccinic acid (IDS) as ligand, γ-glycidoxypropyltrimethoxysilane (γ-GLDP) as spacer arm and silica as matrix. The medium was characterized by fourier transform infrared spectrometry (FT-IR). Binding capacity of IDS on chromatographic medium was determined by potentiometric titration. The effect of mass ratios (w/w) between silica and IDS on the medium synthesis was discussed. The optimal ratio of matrix, spacer arm and ligand was 1.5 g: 2.0 mL: 4.6 g. Based on different chromatographic modes, protein mixtures of bovine serum albumin, ribonuclease and lysozyme were successfully separated with IDS-Silica column and IDS-Cu(II)-Silica column, respectively. To examine metal chelating behavior of IDS-Silica stationary phase, optimal geometries and related parameters of complexes formed by IDS with Fe³⁺, Cu²⁺, Ni²⁺, Zn²⁺, Co²⁺ and Ca²⁺ were achieved by quantum computing. The stabilities of the complexes were predicted according to the results of theoretical calculation. Sorption capacities of IDS-Silica stationary phase for metal ions were determined by using offline frontal chromatography (FC) combined with inductively coupled plasma atomic emission spectrometry (ICP-AES). A good agreement was found between simulating results and experimental observations. The order of chelating strength of IDS-Silica medium for metal ions was Fe³⁺ > Cu²⁺ > Ni²⁺ > Zn²⁺ > Co²⁺ > Ca²⁺. Compared with IDA-Silica, Asp-Silica and Glu-Silica stationary phases, IDS-Silica displayed a stronger chelating property for metal ion.

在这项研究中，我们以亚氨基二琥珀酸（IDS）为配体，以γ-环氧丙氧基丙基三甲氧基硅烷（γ-GLDP）为间隔臂，以二氧化硅为基质，开发了一种多功能色谱介质。该介质通过傅立叶变换红外光谱法（FT-IR）表征。通过电位滴定法确定IDS在色谱介质上的结合能力。讨论了二氧化硅和IDS之间的质量比（w / w）对介质合成的影响。基质，间隔臂和配体的最佳比例为1.5 g：2.0 mL：4.6 g。根据不同的色谱模式，分别用IDS-硅胶柱和IDS-Cu（II）-硅胶柱成功分离了牛血清白蛋白，核糖核酸酶和溶菌酶的蛋白质混合物。要检查IDS-二氧化硅固定相的金属螯合行为，通过量子计算获得了³⁺，Cu 2 ⁺，Ni 2 ⁺，Zn 2 ⁺，Co ²⁺和Ca ²⁺。根据理论计算结果预测了配合物的稳定性。IDS-硅胶固定相对金属离子的吸附能力是通过使用离线额叶色谱（FC）结合电感耦合等离子体原子发射光谱法（ICP-AES）来确定的。在模拟结果和实验观察之间发现了很好的一致性。IDS-二氧化硅介质对金属离子的螯合强度顺序为Fe ³⁺  > Cu ²⁺  > Ni ²⁺  > Zn ²⁺  > Co ²⁺ > Ca ²⁺。与IDA-Silica，Asp-Silica和Glu-Silica固定相相比，IDS-Silica对金属离子具有更强的螯合性能。