Photon Antibunching Reveals Static and Dynamic Quenching Interaction of Tryptophan with Atto-655,The Journal of Physical Chemistry Letters

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Photon Antibunching Reveals Static and Dynamic Quenching Interaction of Tryptophan with Atto-655
The Journal of Physical Chemistry Letters ( IF 4.8 ) Pub Date : 2017-11-16 00:00:00 , DOI: 10.1021/acs.jpclett.7b02430
Arjun Sharma _{1,

2} , Jörg Enderlein ₃ , Manoj Kumbhakar _{1,

2}

Affiliation

Fluorescence correlation spectroscopy (FCS) of photoinduced electron transfer (PET) between the dye Atto-655 and the amino acid tryptophan has been extensively used for studying fast conformational dynamics of small disordered peptides and proteins. However, a precise understanding of the quenching mechanism and its exact rates that would explain ensemble as well as single-molecule spectroscopy results is still lacking. In this contribution, a general unified model for intermolecular PET between Atto-655 and tryptophan is developed, which involves ground-state complex formation, quenching sphere of action, and dynamic quenching at the single-molecule level. We present measurements of fluorescence antibunching, fluorescence lifetime, and steady-state fluorescence intensity and absorbance and demonstrate that our model is capable to describe all results in a global and coherent manner.

中文翻译：

光子反聚束揭示了色氨酸与Atto-655的静态和动态猝灭相互作用。

染料Atto-655和氨基酸色氨酸之间的光诱导电子转移（PET）的荧光相关光谱（FCS）已广泛用于研究小无序肽和蛋白质的快速构象动力学。然而，仍然缺乏对淬灭机理及其确切速率的精确理解，这可以解释整体以及单分子光谱学结果。在此贡献中，开发了Atto-655和色氨酸之间分子间PET的通用统一模型，该模型涉及基态复合物的形成，作用域的猝灭和单分子水平的动态猝灭。我们介绍了荧光抗聚束，荧光寿命，

更新日期：2017-11-16

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