Direct mercury determination in blood and urine by means of high-resolution continuum source graphite furnace atomic absorption spectrometry using gold nanoparticles as a chemical modifier,Journal of Analytical Atomic Spectrometry

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Direct mercury determination in blood and urine by means of high-resolution continuum source graphite furnace atomic absorption spectrometry using gold nanoparticles as a chemical modifier
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2017-10-25 00:00:00 , DOI: 10.1039/c7ja00323d
Maite Aramendía _{1,

2,

3,

4,

5} , Ananda Guarda _{4,

5,

6,

7,

8} , Diego Leite _{4,

5,

6,

7,

8} , Martín Resano _{4,

5,

6,

7,

8}

Affiliation

The determination of Hg in biological fluids, such as blood and urine, is important considering its known toxicity to human health. This kind of analysis typically requires the use of expensive instrumentation and/or sample pretreatment, which can be a problem for implementation in laboratories in low- and middle-income countries. In this work, a cost-effective methodology for the direct determination of Hg in blood and urine at low μg L⁻¹ levels by means of high-resolution continuum source graphite furnace atomic absorption spectrometry (HR CS GFAAS) is presented. This method is based on the use of Au nanoparticles (Au NPs) as a modifier, which can be readily synthesized in any basic laboratory. By using this modifier, direct analysis of one sample can be performed in a few minutes without the need for extensive sample pretreatment and calibrating with aqueous standards. Although developed for HR CS GFAAS, this method might be adopted by any laboratory equipped with any GFAAS instrument. With our instrumentation, a characteristic mass of 16 pg and a LOD of 2.3 μg L⁻¹ (for a typical sample volume of 10 μL, although higher volumes can also be analyzed, if required) was obtained for Hg, which is fit for purpose in the context of Hg exposure monitoring as a result of occupational hazards.

中文翻译：

使用金纳米颗粒作为化学改性剂的高分辨率连续谱源石墨炉原子吸收光谱法直接测定血液和尿液中的汞

考虑到已知的对人体健康的毒性，测定血液和尿液等生物流体中的Hg非常重要。这种分析通常需要使用昂贵的仪器和/或样品预处理，这对于在中低收入国家的实验室中实施可能是一个问题。在这项工作中，一种直接测定低μgL ^-1的血液和尿液中Hg的经济有效的方法通过高分辨率连续谱源石墨炉原子吸收光谱法（HR CS GFAAS）给出了高水平。该方法基于使用金纳米颗粒（Au NPs）作为改性剂，可以在任何基础实验室中轻松合成。通过使用这种修饰剂，可以在几分钟内直接分析一个样品，而无需进行大量的样品预处理和水相标定。尽管此方法是针对HR CS GFAAS开发的，但任何配备有GFAAS仪器的实验室都可以采用此方法。使用我们的仪器，特征质量为16 pg，LOD为2.3μgL ^-1 （对于10 µL的典型样品体积，虽然也可以分析更大的体积）的汞含量较高，这适合作为职业危害导致的汞暴露监测的目的。

更新日期：2017-11-16

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