Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: an electrochemical impedance study,Soft Matter

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Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: an electrochemical impedance study
Soft Matter ( IF 2.9 ) Pub Date : 2017-11-06 00:00:00 , DOI: 10.1039/c7sm01539a
Eleftheria Diamanti _{1,

2,

3,

4} , Eduart Gutiérrez-Pineda _{5,

6,

7,

8,

9} , Nikolaos Politakos _{1,

2,

3,

4} , Patrizia Andreozzi _{1,

2,

3,

4} , María José Rodriguez-Presa _{5,

6,

7,

8,

9} , Wolfgang Knoll _{10,

11,

12,

13} , Omar Azzaroni _{5,

6,

7,

8,

9} , Claudio A. Gervasi _{5,

6,

7,

8,

9} , Sergio E. Moya _{1,

2,

3,

4}

Affiliation

Supported membranes on polymer cushions are of fundamental interest as models for cell membranes. The use of polyelectrolyte multilayers (PEMs) assembled by the layer by layer (LbL) technique as supports for a bilayer allows for easy integration of the lipid bilayer on surfaces and devices and for nanoscale tunable spacing of the lipid bilayer. Controlling ionic permeability in lipid bilayers supported on PEMs triggers potential applications in sensing and as models for transport phenomena in cell membranes. Lipid bilayers displaying gramicidin channels are fabricated on top of polyallylamine hydrochloride (PAH) and polystyrene sulfonate (PSS) multilayer films, by the assembly of vesicles of phosphatidylcholine and phosphatidylserine, 50 : 50 M/M, carrying gramicidin (GA). Quartz crystal microbalance with dissipation shows that the vesicles with GA fuse into a bilayer. Atomic force microscopy reveals that the presence of GA alters the bilayer topography resulting in depressions in the bilayer of around 70 nm in diameter. Electrochemical impedance spectroscopy (EIS) studies show that supported bilayers carrying GA have smaller resistances than the bilayers without GA. Lipid layers carrying GA display a higher conductance for K⁺ than for Na⁺ and are blocked in the presence of Ca²⁺.

中文翻译：

载于聚电解质多层膜上的脂质双层膜中的Gramicidin离子通道：电化学阻抗研究

聚合物垫层上的支撑膜作为细胞膜的模型具有根本的意义。通过逐层（LbL）技术组装的聚电解质多层（PEM）作为双层的支撑物，可以使脂质双层在表面和设备上轻松整合，并实现脂质双层的纳米级可调间距。控制PEM上支持的脂质双层中的离子渗透性可触发传感方面的潜在应用，并可作为细胞膜中运输现象的模型。通过以50:50 M / M的速度携带载有短杆菌肽（GA）的磷脂酰胆碱和磷脂酰丝氨酸的囊泡组装，在聚烯丙胺盐酸盐（PAH）和聚苯乙烯磺酸盐（PSS）多层膜的顶部制备显示出短杆菌肽通道的脂质双层。具有耗散的石英晶体微天平显示，带有GA的囊泡融合成双层。原子力显微镜显示，GA的存在改变了双层形貌，导致双层中直径约70 nm的凹陷。电化学阻抗谱（EIS）研究表明，带有GA的支撑双层比没有GA的双层具有更小的电阻。带有GA的脂质层显示出更高的K电导率⁺比的Na⁺和被阻挡在存在Ca²⁺。

更新日期：2017-11-16

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