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Flat Drops, Elastic Sheets, and Microcapsules by Interfacial Assembly of a Bacterial Biofilm Protein, BslA
Langmuir ( IF 3.7 ) Pub Date : 2017-11-15 00:00:00 , DOI: 10.1021/acs.langmuir.7b03226 Gilad Kaufman 1 , Wei Liu 1 , Danielle M. Williams 1 , Youngwoo Choo 1 , Manesh Gopinadhan 1 , Niveditha Samudrala 1 , Raphael Sarfati 1 , Elsa C. Y. Yan 1 , Lynne Regan 1 , Chinedum O. Osuji 1
Langmuir ( IF 3.7 ) Pub Date : 2017-11-15 00:00:00 , DOI: 10.1021/acs.langmuir.7b03226 Gilad Kaufman 1 , Wei Liu 1 , Danielle M. Williams 1 , Youngwoo Choo 1 , Manesh Gopinadhan 1 , Niveditha Samudrala 1 , Raphael Sarfati 1 , Elsa C. Y. Yan 1 , Lynne Regan 1 , Chinedum O. Osuji 1
Affiliation
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Protein adsorption and assembly at interfaces provide a potentially versatile route to create useful constructs for fluid compartmentalization. In this context, we consider the interfacial assembly of a bacterial biofilm protein, BslA, at air–water and oil–water interfaces. Densely packed, high modulus monolayers form at air–water interfaces, leading to the formation of flattened sessile water drops. BslA forms elastic sheets at oil–water interfaces, leading to the production of stable monodisperse oil-in-water microcapsules. By contrast, water-in-oil microcapsules are unstable but display arrested rather than full coalescence on contact. The disparity in stability likely originates from a low areal density of BslA hydrophobic caps on the exterior surface of water-in-oil microcapsules, relative to the inverse case. In direct analogy with small molecule surfactants, the lack of stability of individual water-in-oil microcapsules is consistent with the large value of the hydrophilic–lipophilic balance (HLB number) calculated based on the BslA crystal structure. The occurrence of arrested coalescence indicates that the surface activity of BslA is similar to that of colloidal particles that produce Pickering emulsions, with the stability of partially coalesced structures ensured by interfacial jamming. Micropipette aspiration and flow in tapered capillaries experiments reveal intriguing reversible and nonreversible modes of mechanical deformation, respectively. The mechanical robustness of the microcapsules and the ability to engineer their shape and to design highly specific binding responses through protein engineering suggest that these microcapsules may be useful for biomedical applications.
中文翻译：
细菌生物膜蛋白BslA的界面组装产生的扁平液滴，弹性片和微胶囊
蛋白质在界面处的吸附和组装提供了一种潜在的通用途径，以创建有用的构建体来进行液体区分开。在这种情况下，我们考虑了细菌生物膜蛋白BslA在空气-水和油-水界面处的界面组装。在空气-水界面形成密集堆积的高模量单层，导致扁平无柄水滴的形成。BslA在油水界面形成弹性片，从而导致稳定的单分散水包油微胶囊的生产。相比之下，油包水型微囊不稳定，但在接触时表现出阻滞而不是完全聚结。相对于相反的情况，稳定性的差异可能是由于油包水型微胶囊外表面的BslA疏水性帽的面密度低所致。与小分子表面活性剂直接类比，单个油包水微胶囊缺乏稳定性与根据BslA晶体结构计算的亲水亲脂平衡值（HLB值）大有关。停滞聚结的出现表明BslA的表面活性类似于产生Pickering乳液的胶体颗粒的表面活性，并且通过界面干扰确保了部分聚结的结构的稳定性。锥形毛细管实验中的微量移液器抽吸和流动分别揭示了有趣的可逆和不可逆机械变形模式。
更新日期：2017-11-16
中文翻译：
细菌生物膜蛋白BslA的界面组装产生的扁平液滴，弹性片和微胶囊
蛋白质在界面处的吸附和组装提供了一种潜在的通用途径，以创建有用的构建体来进行液体区分开。在这种情况下，我们考虑了细菌生物膜蛋白BslA在空气-水和油-水界面处的界面组装。在空气-水界面形成密集堆积的高模量单层，导致扁平无柄水滴的形成。BslA在油水界面形成弹性片，从而导致稳定的单分散水包油微胶囊的生产。相比之下，油包水型微囊不稳定，但在接触时表现出阻滞而不是完全聚结。相对于相反的情况，稳定性的差异可能是由于油包水型微胶囊外表面的BslA疏水性帽的面密度低所致。与小分子表面活性剂直接类比，单个油包水微胶囊缺乏稳定性与根据BslA晶体结构计算的亲水亲脂平衡值（HLB值）大有关。停滞聚结的出现表明BslA的表面活性类似于产生Pickering乳液的胶体颗粒的表面活性，并且通过界面干扰确保了部分聚结的结构的稳定性。锥形毛细管实验中的微量移液器抽吸和流动分别揭示了有趣的可逆和不可逆机械变形模式。
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