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Lipogels for Encapsulation of Hydrophilic Proteins and Hydrophobic Small Molecules
Biomacromolecules ( IF 5.5 ) Pub Date : 2017-12-15 00:00:00 , DOI: 10.1021/acs.biomac.7b01300
Celia C Homyak , Ann Fernandez , Mollie A Touve 1 , Bo Zhao , Francesca Anson , Jeanne A Hardy , Richard W Vachet , Nathan C Gianneschi 1, 2 , Jennifer L Ross , S Thayumanavan
Affiliation  

Lipid–polymer hybrid materials have the potential to exhibit enhanced stability and loading capabilities in comparison to parent liposome or polymer materials. However, complexities lie in formulating and characterizing such complex nanomaterials. Here we describe a lipid-coated polymer gel (lipogel) formulated using a single-pot methodology, where self-assembling liposomes template a UV-curable polymer gel core. Using fluorescently labeled lipids, protein, and hydrophobic molecules, we characterized their formation, purification, stability, and encapsulation efficiency via common instrumentation methods such as dynamic light scattering (DLS), matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS), UV–vis spectroscopy, fluorescence spectroscopy, and single-particle total internal reflection fluorescence (TIRF) microscopy. In addition, we confirmed that these dual-guest-loaded lipogels are stable in solution for several months. The simplicity of this complete aqueous formation and noncovalent dual-guest encapsulation holds potential as a tunable nanomaterial scaffold.

中文翻译:

用于封装亲水性蛋白质和疏水性小分子的脂质体

与母体脂质体或聚合物材料相比,脂质-聚合物杂化材料有可能表现出增强的稳定性和负载能力。然而,这种复杂纳米材料的配制和表征很复杂。在这里,我们描述了一种使用单罐方法配制的脂质涂层聚合物凝胶(lipogel),其中自组装脂质体模板为紫外线固化聚合物凝胶核心。使用荧光标记的脂质、蛋白质和疏水性分子,我们通过动态光散射 (DLS)、基质辅助激光解吸电离质谱 (MALDI-MS) 等常用仪器方法表征了它们的形成、纯化、稳定性和封装效率、紫外可见光谱、荧光光谱和单颗粒全内反射荧光 (TIRF) 显微镜。此外,我们还证实这些双客体负载的脂质凝胶在溶液中可以稳定几个月。这种完全水性形成和非共价双客体封装的简单性具有作为可调纳米材料支架的潜力。
更新日期:2017-12-15
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