当前位置：
X-MOL 学术
›
Anal. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A Target-Triggered DNAzyme Motor Enabling Homogeneous, Amplified Detection of Proteins
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-11-15 00:00:00 , DOI: 10.1021/acs.analchem.7b03529 Junbo Chen 1, 2 , Albert Zuehlke 2 , Bin Deng 2 , Hanyong Peng 2 , Xiandeng Hou 1, 3 , Hongquan Zhang 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-11-15 00:00:00 , DOI: 10.1021/acs.analchem.7b03529 Junbo Chen 1, 2 , Albert Zuehlke 2 , Bin Deng 2 , Hanyong Peng 2 , Xiandeng Hou 1, 3 , Hongquan Zhang 2
Affiliation
|
We report here the concept of a self-powered, target-triggered DNA motor constructed by engineering a DNAzyme to adapt into binding-induced DNA assembly. An affinity ligand was attached to the DNAzyme motor via a DNA spacer, and a second affinity ligand was conjugated to the gold nanoparticle (AuNP) that was also decorated with hundreds of substrate strands serving as a high-density, three-dimensional track for the DNAzyme motor. Binding of a target molecule to the two ligands induced hybridization between the DNAzyme and its substrate on the AuNP, which are otherwise unable to spontaneously hybridize. The hybridization of DNAzyme with the substrate initiates the cleavage of the substrate and the autonomous movement of the DNAzyme along the AuNP. Each moving step restores the fluorescence of a dye molecule, enabling monitoring of the operation of the DNAzyme motor in real time. A simple addition or depletion of the cofactor Mg2+ allows for fine control of the DNAzyme motor. The motor can translate a single binding event into cleavage of hundreds of substrates, enabling amplified detection of proteins at room temperature without the need for separation.
中文翻译：
目标触发的DNAzyme电机，实现蛋白质的均质，放大检测
我们在这里报告了通过工程化DNA酶以适应结合诱导的DNA装配而构建的自供电，目标触发的DNA电动机的概念。亲和配体通过DNA间隔子连接到DNAzyme马达，第二个亲和配体与金纳米颗粒（AuNP）缀合，金纳米颗粒也用数百个底物链修饰，用作高密度的三维轨道DNAzyme马达。靶分子与两个配体的结合引起DNA核酶与其在AuNP上的底物之间的杂交，否则该杂交不能自发杂交。DNAzyme与底物的杂交引发了底物的裂解以及DNAzyme沿着AuNP的自主运动。每个移动步骤都会还原染料分子的荧光，可以实时监控DNAzyme电机的运行情况。辅助因子Mg的简单添加或消耗2+可实现对DNAzyme马达的精细控制。该马达可以将单个结合事件转化为数百种底物的裂解，从而无需分离即可在室温下放大检测蛋白质。
更新日期：2017-11-16
中文翻译：
目标触发的DNAzyme电机，实现蛋白质的均质，放大检测
我们在这里报告了通过工程化DNA酶以适应结合诱导的DNA装配而构建的自供电，目标触发的DNA电动机的概念。亲和配体通过DNA间隔子连接到DNAzyme马达，第二个亲和配体与金纳米颗粒（AuNP）缀合，金纳米颗粒也用数百个底物链修饰，用作高密度的三维轨道DNAzyme马达。靶分子与两个配体的结合引起DNA核酶与其在AuNP上的底物之间的杂交，否则该杂交不能自发杂交。DNAzyme与底物的杂交引发了底物的裂解以及DNAzyme沿着AuNP的自主运动。每个移动步骤都会还原染料分子的荧光，可以实时监控DNAzyme电机的运行情况。辅助因子Mg的简单添加或消耗2+可实现对DNAzyme马达的精细控制。该马达可以将单个结合事件转化为数百种底物的裂解，从而无需分离即可在室温下放大检测蛋白质。
京公网安备 11010802027423号