The mechanical scouring of bio-carriers improves phosphorus removal and mediates functional microbiomes in membrane bioreactors†,Environmental Science: Water Research & Technology

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The mechanical scouring of bio-carriers improves phosphorus removal and mediates functional microbiomes in membrane bioreactors†
Environmental Science: Water Research & Technology ( IF 3.5 ) Pub Date : 2017-11-15 00:00:00 , DOI: 10.1039/c7ew00385d
Ronghua Xu _{1,

2,

3,

4,

5} , Zhongbo Zhou _{1,

2,

3,

4,

5} , Fangang Meng _{1,

2,

3,

4,

5}

Affiliation

Integrated fixed-film activated sludge combined with a membrane bioreactor (IFAS-MBR) is a promising alternative to improve biological nutrient removal (BNR). The synergistic benefits of the attached-growth biofilm on reactor performance have been extensively reported, yet the effects of moving carriers on driving changes in the bulk sludge bacterial community are poorly documented. In this work, two parallel MBRs (i.e., with and without carriers) were operated to determine the roles of moving carriers in shifting the bacterial community composition, particularly the active microbiota, and their impact on BNR. The results showed that the presence of moving carriers significantly improved phosphorus removal (p < 0.01) during stable operation, which was in good agreement with the higher specific phosphorus release/uptake rates of the bulk sludge from the carrier-dosed MBR compared to that from the control MBR determined by a series of batch tests. LIVE/DEAD BacLight viability staining and the use of propidium monoazide (PMA) together with 16S rRNA amplicon sequencing indicated that the bulk sludge in the carrier-dosed MBR had a higher viability and biodiversity within the active bacterial community compared with that in the control MBR, attributable to the mechanically induced decrease in floc size and an increase in mass transfer. Notably, a higher abundance of phosphate-accumulating organisms (PAOs) and lower GAOs/PAO ratio (GAOs: glycogen-accumulating organisms) were observed in the active microbiota of the carrier-dosed MBR than in the control MBR. By contrast, the abundance of ammonia oxidizing bacteria, nitrite oxidizing bacteria and denitrifiers was comparable in both MBRs. Intriguingly, these functional bacteria had highly divergent abundances between the total and active bacterial communities, likely attributable to their different susceptibilities to oxygen and substrates. Overall, this study substantially extends our understanding of the roles of fluidized particles in mediating microbiota changes in an IFAS-MBR system.

中文翻译：

对生物载体的机械洗涤可改善除磷能力，并介导膜生物反应器中的功能微生物群†

结合了膜生物反应器（IFAS-MBR）的固定膜活化污泥是提高生物营养去除率（BNR）的有前途的替代方法。附着生长的生物膜对反应器性能的协同增效作用已得到广泛报道，但移动载剂对推动整体污泥细菌群落变化的影响却鲜有文献报道。在这项工作中，操作了两个平行的MBR（即带有和不带有载体的MBR ），以确定移动的载体在改变细菌群落组成（特别是活性菌群）中的作用及其对BNR的影响。结果表明，移动载流子的存在显着改善了除磷（p<0.01）在稳定运行期间，这与通过一系列分批测试确定的从控制型MBR运出的污泥的较高的比磷释放/吸收速率相比，与通过载体MBR进行的污泥的更高的比磷释放/吸收率非常吻合。LIVE / DEAD BacLight活力染色以及单叠氮化丙锭（PMA）的使用以及16S rRNA扩增子测序表明，与对照MBR相比，载体给药的MBR中活性细菌群落中的大部分污泥具有更高的活力和生物多样性。，这归因于机械诱导的絮凝物尺寸减小和传质增加。值得注意的是，磷酸盐积累生物（PAOs）的含量较高，而GAOs / PAO比值较低（GAOs：与对照MBR相比，在以载剂为主的MBR的活性菌群中观察到了糖原积累生物）。相比之下，两种MBR的氨氧化细菌，亚硝酸盐氧化细菌和反硝化剂的含量都相当。有趣的是，这些功能性细菌在总细菌和活性细菌群落之间的丰度差异很大，这很可能是由于它们对氧气和底物的敏感性不同。总的来说，这项研究大大扩展了我们对流化颗粒在介导IFAS-MBR系统中微生物群变化中的作用的理解。这些功能性细菌在总细菌和活跃细菌群落之间的丰度差异很大，这很可能是由于它们对氧气和底物的敏感性不同。总的来说，这项研究大大扩展了我们对流化颗粒在介导IFAS-MBR系统中微生物群变化中的作用的理解。这些功能性细菌在总细菌和活跃细菌群落之间的丰度差异很大，这很可能是由于它们对氧气和底物的敏感性不同。总的来说，这项研究大大扩展了我们对流化颗粒在介导IFAS-MBR系统中微生物群变化中的作用的理解。

更新日期：2017-11-15

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