Dengue virus (DENV), a single-strand positive-sense RNA virus, is transmitted by mosquitos and can cause severe disease. DENV has four closely related antigenically distinct serotypes, DENV-1 to DENV-4, which differ by 25 to 40% at the amino acid level. Thai et al. (K. T. D. Thai et al., J Virol 86:835–843, 2012, https://doi.org/10.1128/JVI.05985-11) investigated intrahost DENV variation to determine the extent and pattern of genetic diversity. Sequence data in domain III of the envelope gene was obtained from serial plasma samples (n = 49) taken from 17 patients infected with DENV-1, totaling some 8,458 clones. Nucleotide sequence diversities of viral populations were very low, with conservative estimates of the average levels of genetic diversity ranging from 0 to 0.0013. However, there was clear evidence for mixed infection, with the presence of multiple phylogenetically distinct lineages present within the same host. However, no relationship was observed between the extent and pattern of DENV-1 genetic diversity and disease severity, immune status, or level of viremia.

登革热病毒（DENV）是一种单链正义RNA病毒，由蚊子传播，可导致严重疾病。DENV具有四种密切相关的抗原性不同的血清型，DENV-1至DENV-4，在氨基酸水平上相差25％至40％。Thai等。（KTD Thai et al。，J Virol 86：835–843，2012，https ://doi.org/10.1128/JVI.05985-11 ）研究了宿主内DENV变异以确定遗传多样性的程度和模式。包膜基因III结构域的序列数据是从连续血浆样品中获得的（n= 49）来自17名感染DENV-1的患者，共约8,458个克隆。病毒种群的核苷酸序列多样性非常低，保守估计遗传多样性的平均水平在0到0.0013之间。但是，有明确的证据表明混合感染，同一宿主内存在多个系统发育上不同的谱系。但是，在DENV-1遗传多样性的程度和模式与疾病的严重程度，免疫状态或病毒血症水平之间未发现任何关系。