A simple isocratic HPLC method using visible detection was developed and validated for the quantification of gold in nanoparticles (AuNP). After a first step of oxidation of nanoparticles, an ion-pair between tetrachloroaurate anion and the cationic dye Rhodamine B was formed and extracted from the aqueous media with the help of an organic solvent. The corresponding Rhodamine B was finally quantified by reversed phase liquid chromatography using a Nucleosil C18 (150 mm × 4.6 mm, 3 µm) column and with a mobile phase containing acetonitrile and 0.1% trifluoroacetic acid aqueous solution (25/75, V/V) at 1.0 mL min^−1.and at a wavelength of 555 nm. The method was validated using methodology described by the International Conference on Harmonization and was shown to be specific, precise (RSD < 11%), accurate and linear in the range of 0.1 – 30.0 µM with a lower limit of quantification (LLOQ) of 0.1 µM. This method was in a first time applied to AuNP quality control after their synthesis. In a second time, the absence of gold leakage (either as AuNP or gold salt form) from nanostructured multilayered polyelectrolyte films under shear stress was assessed.

开发了一种使用可见光检测的简单等度HPLC方法，并验证了该方法用于定量纳米颗粒（AuNP）中的金。在第一步氧化纳米颗粒之后，形成四氯金酸根阴离子和阳离子染料若丹明B之间的离子对，并借助有机溶剂从水性介质中萃取出来。最后，使用Nucleosil C18（150 mm×4.6 mm，3 µm）色谱柱和含有乙腈和0.1％三氟乙酸水溶液（25/75，V / V）的流动相通过反相液相色谱法对相应的若丹明B进行定量。在1.0 mL min ^-1下。波长为555 nm。该方法已使用国际协调会议描述的方法进行了验证，结果显示在0.1 – 30.0 µM的范围内特异性，精确（RSD <11％），准确和线性，定量下限（LLOQ）为0.1 µM。合成后，该方法首次应用于AuNP质量控制。在第二次中，评估了在剪切应力下纳米结构的多层聚电解质膜没有金泄漏（以AuNP或金盐形式）。