Photo-crosslinked mono-component type II collagen hydrogel as a matrix to induce chondrogenic differentiation of bone marrow mesenchymal stem cells,Journal of Materials Chemistry B

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Photo-crosslinked mono-component type II collagen hydrogel as a matrix to induce chondrogenic differentiation of bone marrow mesenchymal stem cells
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-10-19 00:00:00 , DOI: 10.1039/c7tb02348k
Ke Yang _{1,

2,

3,

4} , Jing Sun _{1,

2,

3,

4} , Dan Wei _{1,

2,

3,

4} , Lu Yuan _{1,

2,

3,

4} , Jirong Yang _{1,

2,

3,

4} , Likun Guo _{1,

2,

3,

4} , Hongsong Fan _{1,

2,

3,

4} , Xingdong Zhang _{1,

2,

3,

4}

Affiliation

Type II collagen is a prospective chondro-inductive matrix for bone marrow mesenchymal stem cells (BMSCs), a key component of the extracellular matrix of cartilage; however, its application is limited by deficient fibrillogenesis and gelation. Herein, type II collagen methacrylamide (Col-II-MA) was synthesized by an amidation reaction between the ε-amino groups on collagen lysine and methacrylic anhydride to enable photo-crosslinking of the collagen, thus accomplishing a one-step preparation of mono-component type II collagen hydrogel for the first time. BMSCs encapsulated within the Col-II-MA hydrogel exhibited accelerated proliferation and morphological changes that are similar to chondrogenesis, as well as up-regulated expression of chondrogenic genes and remarkable secretion of the cartilaginous matrix. These results demonstrated that this effective synthetic approach facilitated the formation of photo-active type II collagen hydrogel with a well-preserved triple helical conformation, which provides BMSCs with a favorable microenvironment for growth and the essential chondro-inductive matrix for differentiation. Furthermore, the hydrogel is applicable to microfabrication techniques and displays promise for future applications in microscale tissue engineering.

中文翻译：

光交联的单组分II型胶原蛋白水凝胶作为基质诱导骨髓间充质干细胞的软骨分化

II型胶原蛋白是骨髓间充质干细胞（BMSC）的前瞻性软骨诱导基质，BMSC是软骨细胞外基质的关键成分。然而，其应用受到原纤维形成和凝胶化不足的限制。此处，II型胶原蛋白甲基丙烯酰胺（Col-II-MA）是通过胶原蛋白赖氨酸上的ε-氨基与甲基丙烯酸酐之间的酰胺化反应合成的，从而实现胶原蛋白的光交联，从而完成了单首次使用II型成分胶原蛋白水凝胶。包埋在Col-II-MA水凝胶中的BMSC表现出类似于软骨形成的加速增殖和形态变化，以及软骨生成基因的表达上调和软骨基质的显着分泌。这些结果表明，这种有效的合成方法促进了具有良好保存的三螺旋构象的光敏型II型胶原蛋白水凝胶的形成，这为BMSCs提供了有利的微环境生长和分化所需的软骨诱导基质。此外，水凝胶可应用于微细加工技术，并有望在微尺度组织工程中得到进一步的应用。

更新日期：2017-11-15

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