High-affinity binding of carbon nanomaterials with nucleobases, which is still a challenge, is the basis for DNA directed assembly and sensing. In this work, boron and nitrogen co-doped single-layered graphene quantum dots (BN-SGQDs) are designed as a high-affinity platform for nucleic acid detection and imaging in living cells, which has been confirmed by density functional theory (DFT) simulation and experiments. Owing to their excellent absorption and photoluminescence ability, the high quantum yield (QY 36.5%) yellow fluorescent BN-SGQDs could act as an energy donor in the fluorescence resonance energy transfer (FRET) process for nucleic acid detection. Furthermore, this BN-SGQD based sensing platform has been successfully adopted to visualize the dynamic invasion of human immunodeficiency virus (HIV) DNA into HeLa cells. The high-affinity platform has shown potential for biosensing in complicated biological samples.

中文翻译：

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硼和氮共掺杂的单层石墨烯量子点：一个高亲和力的平台，用于通过荧光共振能量转移可视化HIV DNA对活细胞的动态入侵

碳纳米材料与核碱基的高亲和力结合仍然是一项挑战，是DNA定向组装和传感的基础。在这项工作中，硼和氮共掺杂的单层石墨烯量子点（BN-SGQDs）被设计为用于活细胞中核酸检测和成像的高亲和性平台，这已被密度泛函理论（DFT）证实。模拟和实验。由于其出色的吸收和光致发光能力，高量子产率（QY为36.5％）的黄色荧光BN-SGQD可以在用于核酸检测的荧光共振能量转移（FRET）过程中充当能量供体。此外，该基于BN-SGQD的传感平台已成功地用于可视化人类免疫缺陷病毒（HIV）DNA向HeLa细胞的动态侵袭。