Advanced capability of radially aligned fibrous scaffolds coated with polydopamine for guiding directional migration of human mesenchymal stem cells,Journal of Materials Chemistry B

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Advanced capability of radially aligned fibrous scaffolds coated with polydopamine for guiding directional migration of human mesenchymal stem cells
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-10-17 00:00:00 , DOI: 10.1039/c7tb01758h
Young Min Shin _{1,

2,

3,

4,

5} , Hyeok Jun Shin _{6,

7,

8,

9,

10} , Dae-Hyeok Yang _{1,

2,

3,

4,

5} , Young-Joo Koh _{11,

12,

13,

14} , Heungsoo Shin _{6,

7,

8,

9,

10} , Heung Jae Chun _{1,

2,

3,

4,

5}

Affiliation

In a large tissue defect, faster migration of adjacent tissue toward the defect shortens the tissue regeneration time. Little has been explored on guiding of directional migration from all fronts of the defect boundary towards the center in tissue engineering. This paper demonstrates the effect of radially aligned fibrous scaffolds (RAFSs) coated with polydopamine in order to guide directional migration of human mesenchymal stem cells (hMSCs). RAFSs were electrospun using a collector with a set of electrodes, each constructed with a metallic ring and a point. The polydopamine was then coated by dipping the scaffolds in a dopamine solution (PD-RAFS). The RAFSs exhibited radial distribution of the fibers from the peripheral region toward the center, and polydopamine was uniformly coated over the entire surface by presenting characteristics of the aromatic ring from dopamine. When hMSCs were seeded on the scaffolds, cells grew in an elongated form toward the center along the fiber direction. In particular, the polydopamine coating improved adhesion and spreading of hMSCs on the scaffolds while preserving initial cell orientation. The hMSCs migrated toward the center of the scaffolds at the border of the seeded area; it was enhanced in the order of PD-RAFS > RAFS > random fibrous scaffolds. Therefore, PD-RAFSs can be utilized as an alternate scaffold that can lead to fast and directional migration of cells for finally facilitating tissue regeneration.

中文翻译：

涂有聚多巴胺的径向排列纤维支架的先进能力可指导人间充质干细胞的定向迁移

在大的组织缺损中，相邻组织向缺损的更快迁移会缩短组织再生时间。在组织工程中，关于引导从缺损边界的各个方向向中心的定向迁移的研究很少。本文证明了涂覆聚多巴胺的径向排列的纤维支架（RAFS）的作用，以指导人类间充质干细胞（hMSCs）的定向迁移。使用具有一组电极的集电器对RAFS进行电纺丝，每个电极均由金属环和一个尖端构成。然后通过将支架浸入多巴胺溶液（PD-RAFS）中来涂覆聚多巴胺。RAFS表现出从外围区域到中心的纤维的径向分布，通过呈现多巴胺的芳香环的特性，将聚多巴胺均匀地涂覆在整个表面上。当将hMSCs接种在支架上时，细胞以拉长的形式沿着纤维方向朝着中心生长。特别地，聚多巴胺涂层改善了hMSC在支架上的粘附和扩散，同时保留了初始细胞取向。hMSC向种子区域边界的支架中心迁移。按照PD-RAFS> RAFS>随机纤维支架的顺序增强。因此，PD-RAFS可用作替代支架，可导致细胞快速定向转移，最终促进组织再生。细胞沿纤维方向以拉长的形式向中心生长。特别地，聚多巴胺涂层改善了hMSC在支架上的粘附和扩散，同时保留了初始细胞取向。hMSC向种子区域边界的支架中心迁移。按照PD-RAFS> RAFS>随机纤维支架的顺序增强。因此，PD-RAFS可用作替代支架，可导致细胞快速定向转移，最终促进组织再生。细胞沿纤维方向以拉长的形式向中心生长。特别地，聚多巴胺涂层改善了hMSC在支架上的粘附和扩散，同时保留了初始细胞取向。hMSC向种子区域边界的支架中心迁移。按照PD-RAFS> RAFS>随机纤维支架的顺序增强。因此，PD-RAFS可用作替代支架，可导致细胞快速定向转移，最终促进组织再生。随机的纤维支架。因此，PD-RAFS可用作替代支架，可导致细胞快速定向转移，最终促进组织再生。随机的纤维支架。因此，PD-RAFS可用作替代支架，可导致细胞快速定向转移，最终促进组织再生。

更新日期：2017-11-15

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