Vancomycin-conjugated polythiophene for the detection and imaging of Gram-positive bacteria,Journal of Materials Chemistry B

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Vancomycin-conjugated polythiophene for the detection and imaging of Gram-positive bacteria
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-10-16 00:00:00 , DOI: 10.1039/c7tb02061a
Ling Gui Ning _{1,

2,

3,

4,

5} , Shuai Wang _{1,

2,

3,

4,

5} , Xue Feng Hu _{5,

6,

7,

8} , Chang Ming Li _{1,

2,

3,

4,

5} , Li Qun Xu _{1,

2,

3,

4,

5}

Affiliation

Bacterial infections can cause serious health problems. The rapid identification of bacteria plays a vital role in the treatment of bacterial infection at an early stage of the disease. In this work, an active polythiophene derivative containing reactive pentafluorophenyl (PFP) ester pendant groups was prepared via Fe³⁺-catalyzed oxidative polymerization. As far as we know, this is the first report of active polythiophene with reactive PFP ester moieties. The active polythiophene derivative was conjugated with vancomycin and α-methoxy-ω-amino poly(ethylene glycol) (mPEG-NH₂) via a reactive ester–amine reaction, resulting in the formation of water-soluble and fluorescent vancomycin-containing polythiophene (PTPVan). Since vancomycin can selectively interact with Gram-positive bacteria and kill them, the antibacterial properties of PTPVan were evaluated. The detection of Gram-positive bacteria was carried out by observing the color change and the fluorescence response of bacteria upon incubation with PTPVan through the naked eye and a fluorescence spectroscope, respectively. The staining of Gram-positive bacteria was observed using a confocal laser scanning microscope (CLSM).

中文翻译：

万古霉素偶联的聚噻吩用于革兰氏阳性细菌的检测和成像

细菌感染会导致严重的健康问题。在疾病的早期阶段，细菌的快速鉴定在细菌感染的治疗中起着至关重要的作用。在这项工作中，通过Fe ³⁺催化的氧化聚合反应制备了具有反应性五氟苯基（PFP）酯侧基的活性聚噻吩衍生物。据我们所知，这是具有活性PFP酯部分的活性聚噻吩的首次报道。活性聚噻吩衍生物，用万古霉素缀合和α甲氧基ω氨基聚（乙二醇）（MPEG-NH ₂）经由反应性酯胺反应，导致形成水溶性和荧光的含万古霉素的聚噻吩（PTPVan）。由于万古霉素可以选择性地与革兰氏阳性细菌相互作用并杀死它们，因此对PTPVan的抗菌性能进行了评估。革兰氏阳性细菌的检测是通过分别用肉眼和荧光光谱仪观察与PTPVan孵育后细菌的颜色变化和荧光响应来进行的。使用共聚焦激光扫描显微镜（CLSM）观察革兰氏阳性细菌的染色。

更新日期：2017-11-15

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