Rapid synthesis of nitrogen doped carbon dots and their application as a label free sensor array for simultaneous discrimination of multiple proteins,Journal of Materials Chemistry B

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Rapid synthesis of nitrogen doped carbon dots and their application as a label free sensor array for simultaneous discrimination of multiple proteins
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-10-10 00:00:00 , DOI: 10.1039/c7tb02129a
Shenghao Xu _{1,

2,

3,

4,

5} , Zhengzhong Su _{1,

2,

3,

4,

5} , Zhuo Zhang _{5,

6,

7,

8} , Yongyin Nie _{1,

2,

3,

4,

5} , Jun Wang _{1,

2,

3,

4,

5} , Guanglu Ge _{5,

6,

7,

8} , Xiliang Luo _{1,

2,

3,

4,

5}

Affiliation

In this work, we develop a facile one-step strategy for rapidly (within 1 min) preparing four kinds of nitrogen doped carbon dots (N-CDs) and construct a label free sensor array for protein discrimination. The monodispersed blue emitting N-CDs, with a quantum yield (QY) of about 7.6% (calibrated with quinine sulfate as the reference), were systematically characterized by using fluorescence spectroscopy, atomic force microscopy (AFM), dynamic light scattering (DLS), X-ray photoelectron spectroscopy (XPS) and infrared spectroscopy (IR). Based on the diverse fluorescence intensity response patterns, eight proteins with diverse isoelectric points and molecular weights could be simultaneously and effectively discriminated at nanomolar concentration by linear discrimination analysis (LDA). Proteins with different concentrations or protein mixtures can also be well identified. Furthermore, multiple proteins could still be well identified without any overlap in the presence of human urine. Moreover, serums from rectal cancer patients, Alzheimer's disease patients and healthy people could be effectively discriminated, showing great potential in auxiliary clinical diagnosis.

中文翻译：

氮掺杂碳点的快速合成及其作为无标记传感器阵列的应用，可同时区分多种蛋白质

在这项工作中，我们开发了一种简便的单步策略，用于快速（在1分钟内）制备四种氮掺杂碳点（N-CD），并构建用于蛋白质识别的无标记传感器阵列。使用荧光光谱，原子力显微镜（AFM），动态光散射（DLS）系统地表征了具有约7.6％量子产率（QY）（以硫酸奎宁作为参考）的单分散发蓝光N-CD。，X射线光电子能谱（XPS）和红外光谱（IR）。基于不同的荧光强度响应模式，可以通过线性判别分析（LDA）在纳摩尔浓度下同时有效区分8个具有不同等电点和分子量的蛋白质。具有不同浓度的蛋白质或蛋白质混合物也可以很好地鉴定出来。此外，在人尿液存在的情况下，仍然可以很好地鉴定出多种蛋白质而没有任何重叠。而且，可以有效地区分来自直肠癌患者，阿尔茨海默氏病患者和健康人的血清，在辅助临床诊断中显示出巨大的潜力。

更新日期：2017-11-15

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