A new colorimetric and fluorescent probe with a large stokes shift for rapid and specific detection of biothiols and its application in living cells,Journal of Materials Chemistry B

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A new colorimetric and fluorescent probe with a large stokes shift for rapid and specific detection of biothiols and its application in living cells
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-09-27 00:00:00 , DOI: 10.1039/c7tb02323e
Meng-Zhao Zhang _{1,

2,

3,

4,

5} , Hai-Hao Han _{1,

2,

3,

4,

5} , Shao-Ze Zhang _{4,

5,

6,

7,

8} , Cheng-Yun Wang _{1,

2,

3,

4,

5} , Yun-Xiang Lu _{4,

5,

6,

7,

8} , Wei-Hong Zhu _{1,

2,

3,

4,

5}

Affiliation

In this work, a new reversible colorimetric and fluorescent probe for sequential recognition of copper ions and biothiols is synthesized easily. Based on the chelation-enhanced fluorescence quenching (CHEQ) effect, this probe shows high sensitivity and selectivity towards Cu²⁺, which can be detected by the naked eye. And this experimental phenomenon can also be realised upon addition of biothiols, restoring their initial fluorescence intensity. This probe also features a very high response speed (less than 5 seconds) and a large stokes shift (178 nm) toward Cu²⁺ and biothiols. Moreover, the detection limit for Cu²⁺ and biothiols is as low as 7.34 nM and 10.3 nM, respectively. Additionally, this ON–OFF–ON-type fluorescence recognition cycle can be repeated more than 5 times by addition of Cu²⁺ and biothiols in turn. Particularly, this 1–Cu²⁺ ensemble is further successfully applied for GSH detection in living cells.

中文翻译：

一种新型的比色荧光探针，具有较大的斯托克斯位移，可快速，特异性地检测生物硫醇及其在活细胞中的应用

在这项工作中，可以轻松合成用于顺序识别铜离子和生物硫醇的新型可逆比色和荧光探针。基于螯合增强的荧光猝灭（CHEQ）效应，该探针显示出对Cu ^2+的高灵敏度和选择性，可以用肉眼检测到。通过添加生物硫醇，恢复其初始荧光强度，也可以实现这种实验现象。该探针还具有非常高的响应速度（不到5秒），并且向Cu ²⁺和生物硫醇的冲程较大（178 nm）。此外，Cu ²⁺的检出限生物硫醇分别低至7.34 nM和10.3 nM。此外，通过依次添加Cu ²⁺和生物硫醇，可以将这种ON-OFF-ON型荧光识别循环重复5次以上。特别是，这种1 -Cu ²⁺集成体进一步成功地用于活细胞中GSH的检测。

更新日期：2017-11-15

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