N-Cadherin modified lipid bilayers promote neural network formation and circuitry,Soft Matter

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N-Cadherin modified lipid bilayers promote neural network formation and circuitry
Soft Matter ( IF 2.9 ) Pub Date : 2017-10-13 00:00:00 , DOI: 10.1039/c7sm01214d
K. Zobel _{1,

2,

3,

4} , S. E. Choi _{1,

2,

3,

4} , R. Minakova _{1,

2,

3,

4} , M. Gocyla _{1,

2,

3,

4} , A. Offenhäusser _{1,

2,

3,

4}

Affiliation

Neural adhesion, maturation, and the correct wiring of the brain to establish each neuron's intended connectivity are controlled by complex interactions of bioactive molecules such as ligands, growth factors, or enzymes. The correct pairing of adjacent neurons is thought to be highly regulated by ligand-mediated cell–cell adhesion proteins, which are known to induce signaling activities. We developed a new platform consisting of supported lipid bilayers incorporated with Fc-chimera synaptic proteins like ephrinA5 or N-cadherin. We extensively characterized their function employing a quartz crystal microbalance with dissipation (QCM-D), calcium imaging, and immunofluorescence analysis. Our biomimetic platform has been shown to promote neural cell adhesion and to improve neural maturation at day in vitro 7 (DIV7) as indicated by an elevated expression of synaptophysin.

中文翻译：

N-钙黏着蛋白修饰的脂质双层促进神经网络的形成和电路

神经粘附，成熟和正确的大脑连线以建立每个神经元的预期连接性，是由生物活性分子（例如配体，生长因子或酶）的复杂相互作用所控制的。相邻神经元的正确配对被认为是受配体介导的细胞间粘附蛋白高度调节的，已知该蛋白可诱导信号传导活性。我们开发了一个新的平台，该平台由与ephrinA5或N-cadherin等Fc-嵌合体突触蛋白结合的支持的脂质双层组成。我们使用带有耗散的石英晶体微量天平（QCM-D），钙成像和免疫荧光分析对它们的功能进行了广泛的表征。我们的仿生平台已被证明可以促进神经细胞粘附并改善体外一天的神经成熟度如图7（DIV7）所示，突触素的表达升高。

更新日期：2017-11-15

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