This paper reports a multi-throughput multi-organ-on-a-chip system formed on a pneumatic pressure-driven medium circulation platform with a microplate-sized format as a novel type of microphysiological system. The pneumatic pressure-driven platform enabled parallelized multi-organ experiments (i.e. simultaneous operation of multiple multi-organ culture units) and pipette-friendly liquid handling for various conventional cell culture experiments, including cell seeding, medium change, live/dead staining, cell growth analysis, gene expression analysis of collected cells, and liquid chromatography–mass spectrometry analysis of chemical compounds in the culture medium. An eight-throughput two-organ system and a four-throughput four-organ system were constructed on a common platform, with different microfluidic plates. The two-organ system, composed of liver and cancer models, was used to demonstrate the effect of an anticancer prodrug, capecitabine (CAP), whose metabolite 5-fluorouracil (5-FU) after metabolism by HepaRG hepatic cells inhibited the proliferation of HCT-116 cancer cells. The four-organ system, composed of intestine, liver, cancer, and connective tissue models, was used to demonstrate evaluation of the effects of 5-FU and two prodrugs of 5-FU (CAP and tegafur) on multiple organ models, including cancer and connective tissue.

中文翻译：

---

平板格式的气压驱动介质循环平台上的多通量多芯片单系统†

本文报道了一种在微压板大小格式的气压驱动介质循环平台上形成的多通量多芯片单系统，这是一种新型的微生理系统。气压驱动平台可实现并行多器官实验（即可同时操作多个多器官培养单元）和移液器友好的液体处理系统，用于各种常规细胞培养实验，包括细胞接种，培养基更换，活/死染色，细胞生长分析，收集到的细胞的基因表达分析和液相色谱分析–培养基中化合物的质谱分析。在具有不同微流体板的共同平台上构建了八通量的两器官系统和四通量的四器官系统。由肝脏和癌症模型组成的两器官系统用来证明抗癌前药卡培他滨（CAP）的作用，其代谢物5-氟尿嘧啶（5-FU）在HepaRG肝细胞代谢后抑制了HCT的增殖。 -116癌细胞。四器官系统，由肠，肝，癌症，