Near-infrared aza-BODIPY fluorescent probe for selective Cu2+ detection and its potential in living cell imaging†,Dalton Transactions

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Near-infrared aza-BODIPY fluorescent probe for selective Cu2+ detection and its potential in living cell imaging†
Dalton Transactions ( IF 3.5 ) Pub Date : 2017-11-15 00:00:00 , DOI: 10.1039/c7dt03481d
Yordkhuan Tachapermpon _{1,

2,

3,

4,

5} , Sopida Thavornpradit _{1,

2,

3,

4,

5} , Adisri Charoenpanich _{2,

3,

4,

5,

6} , Jitnapa Sirirak _{1,

2,

3,

4,

5} , Kevin Burgess _{1,

7,

8,

9} , Nantanit Wanichacheva _{1,

2,

3,

4,

5}

Affiliation

A near-infrared (NIR) fluorescent sensor 1 composed of an aza-boron-dipyrromethene (aza-BODIPY) core covalently bound to two di-2-picolylamine moieties was conceived for Cu²⁺ detection in aqueous solutions. Spectroscopic properties and binding abilities with several metal ions were investigated in phosphate buffered saline (pH 7.4): acetonitrile (95 : 5 v/v) with Triton X-100 via fluorometric titrations. The fluorescence of sensor 1 was quenched selectively by cupric ions in the presence of alkali- and transition-metal-ions. A detection limit of 13 ppb was measured for this system, and this is significantly lower than permissible levels of Cu²⁺ in drinking water according to the guidelines described by the U.S. Environmental Protection Agency (EPA) and by the World Health Organization (WHO). Application of the sensor in detecting Cu²⁺ in HepG2 cells was demonstrated.

中文翻译：

用于选择性Cu ²⁺检测的近红外aza-BODIPY荧光探针及其在活细胞成像中的潜力†

设想由共价键合到两个二-2-吡啶甲基胺部分上的氮杂硼双吡咯烷（氮杂-BODIPY）核组成的近红外（NIR）荧光传感器1，用于水溶液中的Cu ²⁺检测。通过荧光滴定法在Triton X-100的磷酸盐缓冲盐水（pH 7.4）：乙腈（95：5 v / v）中研究了光谱性质和与几种金属离子的结合能力。在碱金属和过渡金属离子存在下，铜离子选择性地淬灭传感器1的荧光。该系统的检出限为13 ppb，大大低于允许的Cu ²⁺水平根据美国环境保护署（EPA）和世界卫生组织（WHO）所述的指导原则对饮用水中的维生素进行检测。证明了该传感器在检测HepG2细胞中Cu ^2+方面的应用。

更新日期：2017-11-15

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