Simple and rapid colorimetric detection of melanoma circulating tumor cells using bifunctional magnetic nanoparticles,Analyst

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Simple and rapid colorimetric detection of melanoma circulating tumor cells using bifunctional magnetic nanoparticles
Analyst ( IF 3.6 ) Pub Date : 2017-10-26 00:00:00 , DOI: 10.1039/c7an01102d
Junrong Li _{1,

2,

3,

4,

5} , Jing Wang _{1,

2,

3,

4,

5} , Yuling Wang _{6,

7,

8,

9,

10} , Matt Trau _{1,

2,

3,

4,

5}

Affiliation

Circulating tumor cells (CTCs) are a promising biomarker for monitoring cancer metastasis and therapy response. However, the rarity and inherent fragility of CTCs pose great challenges for CTC detection. Currently, CTC detection requires advanced instruments that might be inaccessible in most hospitals or labs. To allow simple and rapid CTC detection, we propose a sensor platform that relies only on the intrinsic properties of magnetic nanoparticles (MNPs) including rapid separation as well as nanozyme activity. The detection of melanoma CTC, which causes most skin cancer-related deaths, is demonstrated based on bifunctional MNPs. The magnetic property of MNPs enables melanoma CTC isolation and enrichment within 5 min from blood. The nanozyme activity of MNPs allows naked-eye detection of melanoma CTC by catalyzing the oxidation of colourless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue coloured products. Melanoma CTC enumeration can be further achieved by UV-vis measurement. Our platform has successfully detected 13 melanoma CTCs per mL within 50 min. We thus believe this platform could find broad applications both in the clinic and in research.

中文翻译：

使用双功能磁性纳米粒子简单快速地比色检测黑色素瘤循环肿瘤细胞

循环肿瘤细胞（CTC）是用于监测癌症转移和治疗反应的有前途的生物标志物。但是，CTC的稀有性和内在的脆弱性对CTC的检测提出了巨大的挑战。当前，CTC检测需要大多数医院或实验室都无法使用的高级仪器。为了实现简单，快速的CTC检测，我们提出了一种传感器平台，该平台仅依赖于磁性纳米颗粒（MNP）的内在特性，包括快速分离以及纳米酶的活性。基于双功能MNP，已证明可检测到导致大多数与皮肤癌相关死亡的黑色素瘤CTC。MNPs的磁性使黑色素瘤CTC能够在5分钟内从血液中分离并富集。MNP的纳米酶活性可通过催化无色3,3'的氧化来肉眼检测黑色素瘤CTC，5,5'-四甲基联苯胺（TMB）成蓝色。可以通过UV-vis测量进一步实现黑素瘤CTC枚举。我们的平台已在50分钟内成功检测到每毫升13个黑色素瘤CTC。因此，我们认为该平台可以在临床和研究中找到广泛的应用。

更新日期：2017-11-15

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