In the present work a Hydrophilic Interaction Liquid Chromatography-tandem Mass Spectrometry (HILIC-MS/MS) method was developed for the efficient separation and quantification of a large number of small polar bioactive molecules in Royal Jelly. The method was validated and provided satisfactory detection sensitivity for 88 components. Quantification was proven to be precise for 64 components exhibiting good linearity, recoveries R% >90% for the majority of analytes and intra- and inter-day precision from 0.14 to 20% RSD. Analysis of 125 fresh royal jelly samples of Greek origin provided useful information on royal jelly’s hydrophilic bioactive components revealing lysine, ribose, proline, melezitose and glutamic acid to be in high abundance. In addition the occurrence of 18 hydrophilic nutrients which have not been reported previously as royal jelly constituents is shown.

在本工作中，开发了一种亲水相互作用液相色谱-串联质谱（HILIC-MS / MS）方法，用于有效分离和定量蜂王浆中的大量小极性生物活性分子。该方法经过验证，可为88个组分提供令人满意的检测灵敏度。事实证明，定量分析对于64个具有良好线性度的组分是精确的，大多数分析物的回收率R％> 90％，日内和日间精度从0.14％到20％RSD。对来自希腊的125份新鲜蜂王浆样品的分析提供了有关蜂王浆亲水性生物活性成分的有用信息，这些成分表明赖氨酸，核糖，脯氨酸，松三糖和谷氨酸含量很高。