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Poly(ω-methoxyalkyl acrylate)s: Nonthrombogenic Polymer Family with Tunable Protein Adsorption
Biomacromolecules ( IF 5.5 ) Pub Date : 2017-11-28 00:00:00 , DOI: 10.1021/acs.biomac.7b01247 Shingo Kobayashi 1 , Miyuki Wakui 2 , Yukihisa Iwata 2 , Masaru Tanaka 1
Biomacromolecules ( IF 5.5 ) Pub Date : 2017-11-28 00:00:00 , DOI: 10.1021/acs.biomac.7b01247 Shingo Kobayashi 1 , Miyuki Wakui 2 , Yukihisa Iwata 2 , Masaru Tanaka 1
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A series of polyacrylates with different n-alkyl side chain lengths (1 to 6, and 12 carbons) and a ω-methoxy terminal group (poly(ω-methoxyalkyl acrylate): PMCxA) were prepared to study their nonthrombogenicity using human platelet adhesion, micro bicinchoninic acid (micro BCA) protein assay, and enzyme-linked immunosorbent assay (ELISA) tests. In all cases, human platelet adhesion was suppressed on the PMCxA-coated substrates, and the number of human platelets adhered to the PMC3A (poly(3-methoxypropyl acrylate))-coated surface was comparable to that of commercialized nonthrombogenic coating agent poly(2-methoxyethyl acrylate) (PMEA, equal to PMC2A). The amount of protein adsorbed onto the PMCxA was investigated by micro BCA using bovine serum albumin (BSA) and human fibrinogen (hFbn), revealing that PMC3A exhibited significantly high resistance to nonspecific BSA adsorption. Additionally, the amount of hFbn adsorbed onto the PMC3A was suppressed to the same extent as PMEA. The exposure degree of platelet adhesion sites in adsorbed hFbn was evaluated using an ELISA test, and the degree on the PMCxA with three to six methylene carbons was comparable to the PMEA. The hydration water structure in the hydrated PMCxA was also characterized using differential scanning calorimetry (DSC). The amount of intermediate water, which is the hydration water molecules that moderately interact with the polymer matrix, was maximum in the PMEA with two methylene run lengths, whereas the amount decreased by increasing the number of methyelnes in the side chain. The amount of adsorbed protein increased with a decrease in the amount of intermediate water, suggesting that the protein adsorption amount is tunable by simply changing the number of methylene carbons in the side chain. The present study revealed that poly(ω-methoxyalkyl acrylate)s are useful for blood-contacting medical devices, and PMC3A is the best mode of PMCxA to apply as an antiprotein adsorption coating agent.
中文翻译：
聚（ω-甲氧基烷基丙烯酸酯）s：具有可调节蛋白质吸附能力的非血栓形成聚合物家族
制备了一系列具有不同正烷基侧链长度（1至6个碳原子和12个碳原子）和一个ω-甲氧基端基的聚丙烯酸酯（聚（ω-甲氧基烷基丙烯酸酯）：PMC x A），以使用人类血小板研究其非血栓形成性粘附力，微双金鸡宁酸（micro BCA）蛋白测定和酶联免疫吸附测定（ELISA）测试。在所有情况下，都可以抑制人血小板在PMC x A涂层基底上的粘附，并且粘附在PMC3A（聚（3-甲氧基丙基丙烯酸酯））表面上的人血小板数量与市售的非血栓形成剂聚（丙烯酸2-甲氧基乙酯）（PMEA，等于PMC2A）。吸附到PMC x上的蛋白质量微型BCA使用牛血清白蛋白（BSA）和人纤维蛋白原（hFbn）对A进行了研究，结果表明PMC3A对非特异性BSA吸附表现出很高的抵抗力。另外，吸附到PMC3A上的hFbn的量被抑制到与PMEA相同的程度。使用ELISA试验评估吸附的hFbn中血小板粘附位点的暴露程度，在具有3至6个亚甲基碳的PMC x A上的暴露程度与PMEA相当。水合PMC中的水合水结构x还使用差示扫描量热法（DSC）对A进行了表征。中间水的量（与聚合物基质适度相互作用的水合水分子）在具有两个亚甲基游程长度的PMEA中最大，而通过增加侧链中的甲炔数量而减少了。蛋白质的吸附量随着中间水量的减少而增加，这表明通过简单地改变侧链中亚甲基碳的数量就可以调节蛋白质的吸附量。本研究表明，聚（ω-甲氧基烷基丙烯酸酯）可用于与血液接触的医疗设备，而PMC3A是PMC x A用作抗蛋白质吸附涂层剂的最佳方式。
更新日期：2017-11-28
中文翻译：
聚（ω-甲氧基烷基丙烯酸酯）s：具有可调节蛋白质吸附能力的非血栓形成聚合物家族
制备了一系列具有不同正烷基侧链长度（1至6个碳原子和12个碳原子）和一个ω-甲氧基端基的聚丙烯酸酯（聚（ω-甲氧基烷基丙烯酸酯）：PMC x A），以使用人类血小板研究其非血栓形成性粘附力，微双金鸡宁酸（micro BCA）蛋白测定和酶联免疫吸附测定（ELISA）测试。在所有情况下，都可以抑制人血小板在PMC x A涂层基底上的粘附，并且粘附在PMC3A（聚（3-甲氧基丙基丙烯酸酯））表面上的人血小板数量与市售的非血栓形成剂聚（丙烯酸2-甲氧基乙酯）（PMEA，等于PMC2A）。吸附到PMC x上的蛋白质量微型BCA使用牛血清白蛋白（BSA）和人纤维蛋白原（hFbn）对A进行了研究，结果表明PMC3A对非特异性BSA吸附表现出很高的抵抗力。另外，吸附到PMC3A上的hFbn的量被抑制到与PMEA相同的程度。使用ELISA试验评估吸附的hFbn中血小板粘附位点的暴露程度，在具有3至6个亚甲基碳的PMC x A上的暴露程度与PMEA相当。水合PMC中的水合水结构x还使用差示扫描量热法（DSC）对A进行了表征。中间水的量（与聚合物基质适度相互作用的水合水分子）在具有两个亚甲基游程长度的PMEA中最大，而通过增加侧链中的甲炔数量而减少了。蛋白质的吸附量随着中间水量的减少而增加，这表明通过简单地改变侧链中亚甲基碳的数量就可以调节蛋白质的吸附量。本研究表明，聚（ω-甲氧基烷基丙烯酸酯）可用于与血液接触的医疗设备，而PMC3A是PMC x A用作抗蛋白质吸附涂层剂的最佳方式。
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