Multiplexed bioimaging systems have triggered the development of effective assays, contributing new biological information. Although electrochemical imaging is beneficial for quantitative analysis in real time, monitoring multiple cell functions is difficult. We have developed a novel electrochemical imaging system, herein, using a large-scale integration (LSI)-based amperometric device for detecting multiple biomolecules simultaneously. This system is designated as an electrochemicolor imaging system in which the current signals from two different types of biomolecules are depicted as a multicolor electrochemical image. The mode-selectable function of the 400-electrode device enables the imaging system and two different potentials can be independently applied to the selected electrodes. The imaging system is successfully applied for detecting multiple cell functions of the embryonic stem (ES) cell and the rat pheochromocytoma (PC12) cell aggregates. To the best of our knowledge, this is the first time that a real-time electrochemical mapping technique for multiple electroactive species, simultaneously, has been reported. The imaging system is a promising bioanalytical method for exploring complex biological phenomena.

中文翻译：

---

使用基于LSI的设备进行多细胞分析的电化学彩色成像

多重生物成像系统已经触发了有效测定方法的发展，为新的生物学信息做出了贡献。尽管电化学成像对实时定量分析是有益的，但是监测多个细胞功能却很困难。我们已经在这里开发了一种新颖的电化学成像系统，该系统使用基于大规模集成（LSI）的安培设备同时检测多个生物分子。该系统被称为电化学彩色成像系统，其中来自两种不同类型的生物分子的电流信号被描绘为多色电化学图像。400电极设备的模式选择功能使成像系统能够将两个不同的电势独立地施加到选定的电极上。该成像系统已成功应用于检测胚胎干（ES）细胞和大鼠嗜铬细胞瘤（PC12）细胞聚集体的多种细胞功能。据我们所知，这是首次首次报道了同时用于多种电活性物质的实时电化学作图技术。成像系统是探索复杂生物现象的一种有前途的生物分析方法。