Repair of a Site-Specific DNA Cleavage: Old-School Lessons for Cas9-Mediated Gene Editing,ACS Chemical Biology

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Repair of a Site-Specific DNA Cleavage: Old-School Lessons for Cas9-Mediated Gene Editing
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2017-11-14 00:00:00 , DOI: 10.1021/acschembio.7b00760
Danielle N. Gallagher ₁ , James E. Haber ₁

Affiliation

CRISPR/Cas9-mediated gene editing may involve nonhomologous end-joining to create various insertion/deletions (indels) or may employ homologous recombination to modify precisely the target DNA sequence. Our understanding of these processes has been guided by earlier studies using other site-specific endonucleases, both in model organisms such as budding yeast and in mammalian cells. We briefly review what has been gleaned from such studies using the HO and I-SceI endonucleases and how these findings guide current gene editing strategies.

中文翻译：

修复特定于位点的DNA裂解：Cas9介导的基因编辑的旧课程。

CRISPR / Cas9介导的基因编辑可能涉及非同源末端连接，以创建各种插入/缺失（indels），或可能采用同源重组来精确修饰靶DNA序列。我们对这些过程的理解已被早期在模型生物（如发芽酵母）和哺乳动物细胞中使用其他位点特异性核酸内切酶的研究所指导。我们简要回顾了使用HO和I-SceI核酸内切酶从此类研究中收集到的信息，以及这些发现如何指导当前的基因编辑策略。

更新日期：2017-11-14

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