Non-Neurotoxic Nanodiamond Probes for Intraneuronal Temperature Mapping,ACS Nano

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Non-Neurotoxic Nanodiamond Probes for Intraneuronal Temperature Mapping
ACS Nano ( IF 15.8 ) Pub Date : 2017-11-13 00:00:00 , DOI: 10.1021/acsnano.7b04850
David A. Simpson _{1,

2} , Emma Morrisroe ₃ , Julia M. McCoey ₁ , Alain H. Lombard ₄ , Dulini C. Mendis ₅ , François Treussart ₄ , Liam T. Hall ₁ , Steven Petrou _{2,

3,

6,

7} , Lloyd C. L. Hollenberg _{1,

2,

8}

Affiliation

Optical biomarkers have been used extensively for intracellular imaging with high spatial and temporal resolution. Extending the modality of these probes is a key driver in cell biology. In recent years, the nitrogen-vacancy (NV) center in nanodiamond has emerged as a promising candidate for bioimaging and biosensing with low cytotoxicity and stable photoluminescence. Here we study the electrophysiological effects of this quantum probe in primary cortical neurons. Multielectrode array recordings across five replicate studies showed no statistically significant difference in 25 network parameters when nanodiamonds are added at varying concentrations over various time periods, 12–36 h. The physiological validation motivates the second part of the study, which demonstrates how the quantum properties of these biomarkers can be used to report intracellular information beyond their location and movement. Using the optically detected magnetic resonance from the nitrogen-vacancy defects within the nanodiamonds we demonstrate enhanced signal-to-noise imaging and temperature mapping from thousands of nanodiamond probes simultaneously. This work establishes nanodiamonds as viable multifunctional intraneuronal sensors with nanoscale resolution, which may ultimately be used to detect magnetic and electrical activity at the membrane level in excitable cellular systems.

中文翻译：

用于神经内神经温度测绘的非神经毒性纳米金刚石探针

光学生物标记物已广泛用于具有高空间和时间分辨率的细胞内成像。扩展这些探针的方式是细胞生物学的关键驱动力。近年来，纳米金刚石中的氮空位（NV）中心已成为具有低细胞毒性和稳定的光致发光的生物成像和生物传感的有前途的候选者。在这里，我们研究了这种量子探针在原代皮层神经元中的电生理效应。五个重复研究的多电极阵列记录显示，当在不同时间段（12–36小时）以不同浓度添加纳米金刚石时，在25个网络参数上没有统计学上的显着差异。生理验证激发了研究的第二部分，这证明了这些生物标记物的量子特性如何可用于报告细胞内信息的位置和移动范围以外的信息。使用光学检测到的来自纳米金刚石内部氮空位缺陷的磁共振，我们证明了同时来自数千个纳米金刚石探针的增强的信噪比成像和温度映射。这项工作将纳米金刚石确立为具有纳米级分辨率的可行的多功能神经内神经传感器，最终可用于在可激发细胞系统中检测膜水平的磁和电活动。使用光学检测到的来自纳米金刚石内部氮空位缺陷的磁共振，我们证明了同时来自数千个纳米金刚石探针的增强的信噪比成像和温度映射。这项工作将纳米金刚石确立为具有纳米级分辨率的可行的多功能神经内神经传感器，最终可用于在可激发细胞系统中检测膜水平的磁和电活动。使用光学检测到的来自纳米金刚石内部氮空位缺陷的磁共振，我们证明了同时来自数千个纳米金刚石探针的增强的信噪比成像和温度映射。这项工作将纳米金刚石确立为具有纳米级分辨率的可行的多功能神经内神经传感器，最终可用于在可激发细胞系统中检测膜水平的磁和电活动。

更新日期：2017-11-14

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