Nanoparticle Synthesis, Characterization, and Ecotoxicity: A Research-Based Set of Laboratory Experiments for a General Chemistry Course,Journal of Chemical Education

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Nanoparticle Synthesis, Characterization, and Ecotoxicity: A Research-Based Set of Laboratory Experiments for a General Chemistry Course
Journal of Chemical Education ( IF 2.5 ) Pub Date : 2017-11-13 00:00:00 , DOI: 10.1021/acs.jchemed.7b00369
Zoe N. Amaris ₁ , Daniel N. Freitas ₁ , Karen Mac ₁ , Kyle T. Gerner ₁ , Catherine Nameth ₂ , Korin E. Wheeler ₁

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A series of laboratory experiments were developed to introduce first-year chemistry students to nanoscience through a green chemistry approach. Students made and characterized the stability of silver nanoparticles using two different methods: UV-visible spectroscopy and dynamic light scattering. They then assessed the ecotoxicity of silver nanoparticles in Escherichia coli sublethal growth curve assays. Bacterial growth was monitored via optical density and carbon dioxide measurements. Finally, students designed and implemented their own nanomaterial characterization and ecotoxicity experiments based upon the insights gained in previous tests. The experiments translated current research in sustainable nanomaterials to an undergraduate population while introducing them to research methodology and experiences early in their undergraduate career. Moreover, the experiments emphasized the interdisciplinary nature of the sciences to provide real-world applications and connect concepts learned within general chemistry lecture and lab to other classes commonly taken by first-year STEM students, including biology, materials science, and environmental sciences.

中文翻译：

纳米粒子的合成，表征和生态毒性：基于研究的通用化学课程实验室实验集

开发了一系列实验室实验，以通过绿色化学方法将一年级化学专业的学生介绍给纳米科学。学生使用两种不同的方法制作并表征了银纳米颗粒的稳定性：紫外可见光谱法和动态光散射法。然后他们评估了银纳米颗粒在大肠杆菌中的生态毒性。亚致死生长曲线测定。通过光密度和二氧化碳测量监测细菌的生长。最后，学生根据先前测试中获得的见识，设计并实施了自己的纳米材料表征和生态毒性实验。实验将当前在可持续纳米材料方面的研究成果转化为大学生群体，同时将其介绍给他们在大学生职业生涯初期的研究方法和经验。此外，实验强调科学的跨学科性质，以提供实际应用，并将在普通化学讲座和实验室中学习到的概念与第一年级STEM学生通常参加的其他课程联系起来，包括生物学，材料科学和环境科学。

更新日期：2017-11-13

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