Extracellular polymeric substances (EPS) and ciprofloxacin-degrading microbial community in the combined Fe-C micro-electrolysis and up-flow biological aerated filter (UBAF) process for the treatment of high-level ciprofloxacin (CIP) were analyzed. The research demonstrated a great potential of Fe-C micro-electrolysis-UBAF for the elimination of high-level CIP. Above 90% of CIP removal was achieved through the combined process at 100 mg L⁻¹ of CIP loading. In UBAF, the pollutants were mainly removed at 0–70 cm heights. Three-dimensional fluorescence spectrum (3D-EEM) was used to characterize the chemical structural of loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS) extracted from biofilm sample in UBAF. The results showed that the protein-like substances in LB-EPS and TB-EPS had no clear change in the study. Nevertheless, an obvious release of polysaccharides in EPSs was observed during long-term exposure to CIP, which was considered as a protective response of microbial to CIP toxic. The high-throughput sequencing results revealed that the biodiversity of bacteria community became increasingly rich with gradual ciprofloxacin biodegradation in UBAF. The ciprofloxacin-degrading microbial community was mainly dominated by Proteobacteria and Bacteroidetes. Microorganisms from genera Dechloromonas, Brevundimonas, Flavobacterium, Sphingopyxis and Bosea might take a major role in ciprofloxacin degradation. This study provides deep theoretical guidance for real CIP wastewater treatment.

分析了Fe-C微电解-上流生物曝气滤池（UBAF）联合处理高水平环丙沙星（CIP）的细胞外聚合物（EPS）和环丙沙星降解微生物群落。研究表明，Fe-C微电解UBAF在消除高水平CIP方面具有巨大潜力。通过组合过程在100 mg L ^-1时实现了超过90％的CIP去除的CIP加载。在UBAF中，污染物主要是在0–70 cm的高度处去除的。使用三维荧光光谱（3D-EEM）表征从UBAF中从生物膜样品中提取的松散结合的EPS（LB-EPS）和紧密结合的EPS（TB-EPS）的化学结构。结果表明，LB-EPS和TB-EPS中的蛋白样物质在研究中没有明显变化。尽管如此，在长期暴露于CIP的过程中，仍观察到EPS中多糖的明显释放，这被认为是微生物对CIP毒性的保护反应。高通量测序结果表明，随着UBAF中环丙沙星的逐步生物降解，细菌群落的生物多样性变得越来越丰富。环丙沙星降解微生物群落主要由变形杆菌控制。和拟杆菌。从属微生物脱氯，短波单胞，黄杆菌，鞘脂单胞和氧化包西氏可能采取环丙沙星降解了重要作用。该研究为实际的CIP废水处理提供了深刻的理论指导。