Abstract Leucine-derived norbornene monomer (1), oligo(lactic acid)-derived norborne macromonomer (2) and indomethacin-conjugated norbornene (3) were synthesized and polymerized using the Grubbs ruthenium complex as a catalyst. Macromonomer 2 was synthesized by the ring-opening polymerization of lactide using 5-norbornene-2,3-exo,exo-dimethanol as an initiator and 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) as a catalyst. The corresponding polymers, poly(1), poly(2), poly(3) and copolymers with number-average molecular weights (Mn) ranging from 14 000 to 360 000 were obtained in 73–95% yields. Poly(2) formed scaffolds by simply drying under reduced pressure presumably due to the brush-like structure causing entanglement of the polymer chains. The compressive strength of poly(2) scaffold increased as increasing the polylactide chain length. The compressive strength of the scaffolds obtained from the copolymers ranged from 0.222 to 0.587 MPa in accordance with the monomer unit ratios. SEM revealed the presence of pores around 5–10 µm diameters, together with larger pores dispersed in the range of 50–300 µm diameters. TEM demonstrated the presence of polymer networks. The conjugated indomethacin was gradually released by incubation under acidic condition (pH 5.7) at 37 °C. The results of % cell viability, release of LDH activity and cell morphology assays revealed that the obtained scaffolds did not exhibit cytotoxicity toward HEK 293 cells.

中文翻译：

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大孔支架：基于低聚（乳酸）-氨基酸-吲哚美辛共轭聚（降冰片烯）的分子刷

摘要 以Grubbs钌配合物为催化剂，合成并聚合了亮氨酸衍生的降冰片烯单体(1)、低聚(乳酸)衍生的降冰片烯大单体(2)和吲哚美辛共轭降冰片烯(3)。以 5-降冰片烯-2,3-exo,exo-二甲醇为引发剂，1,8-二氮杂双环[5.4.0]十一碳-7-烯（DBU）为引发剂，通过丙交酯开环聚合合成大单体2催化剂。相应的聚合物，poly(1)、poly(2)、poly(3) 和数均分子量 (Mn) 范围为 14 000 至 360 000 的共聚物的产率为 73-95%。Poly(2) 通过在减压下简单干燥形成支架，这可能是由于刷状结构导致聚合物链缠结。聚(2) 支架的抗压强度随着聚丙交酯链长度的增加而增加。根据单体单元比，由共聚物获得的支架的抗压强度范围为 0.222 至 0.587 MPa。SEM 显示存在大约 5-10 µm 直径的孔，以及分散在 50-300 µm 直径范围内的较大孔。TEM 证明了聚合物网络的存在。通过在酸性条件 (pH 5.7) 下 37 °C 孵育，结合的吲哚美辛逐渐释放。细胞活力百分比、LDH 活性的释放和细胞形态分析的结果表明，所获得的支架对 HEK 293 细胞没有细胞毒性。以及分散在 50-300 µm 直径范围内的较大孔。TEM 证明了聚合物网络的存在。通过在酸性条件 (pH 5.7) 下 37 °C 孵育，结合的吲哚美辛逐渐释放。细胞活力百分比、LDH 活性的释放和细胞形态分析的结果表明，所获得的支架对 HEK 293 细胞没有细胞毒性。以及分散在 50-300 µm 直径范围内的较大孔。TEM 证明了聚合物网络的存在。通过在酸性条件 (pH 5.7) 下 37 °C 孵育，结合的吲哚美辛逐渐释放。细胞活力百分比、LDH 活性的释放和细胞形态分析的结果表明，所获得的支架对 HEK 293 细胞没有细胞毒性。