The bone marrow (BM) microenvironment contains many types of cells and molecules with roles in hematopoiesis, osteogenesis, angiogenesis and metabolism. The spatial distribution of the different bone and BM cell types remains elusive, owing to technical challenges associated with bone imaging. To map nonhematopoietic cells and structures in bone and BM, we performed multicolor 3D imaging of osteoblastic, vascular, perivascular, neuronal and marrow stromal cells, and extracellular-matrix proteins in whole mouse femurs. We analyzed potential interactions between cells and molecules on the basis of colocalization of markers. Our results shed light on the markers expressed by different osteolineage cell types; the heterogeneity of vascular and perivascular cells; the neural subtypes innervating marrow and bone; the diversity of stromal cells; and the distribution of extracellular-matrix components. Our complete imaging data set is available for download and can be used in research in bone biology, hematology, vascular biology, neuroscience and extracellular-matrix biology.

中文翻译：

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非造血骨骼和骨髓细胞与分子的三维图。

骨髓（BM）微环境包含许多类型的细胞和分子，它们在造血，成骨，血管生成和代谢中起作用。由于与骨骼成像相关的技术挑战，不同骨骼和BM细胞类型的空间分布仍然难以捉摸。为了绘制非造血细胞和骨骼和BM中的结构图，我们对整个小鼠股骨中的成骨细胞，血管，血管周，神经元和骨髓基质细胞以及细胞外基质蛋白进行了多色3D成像。我们基于标记的共定位分析了细胞和分子之间的潜在相互作用。我们的结果揭示了由不同骨系细胞类型表达的标志物。血管和血管周围细胞的异质性；支配骨髓和骨骼的神经亚型；基质细胞的多样性；和细胞外基质成分的分布。我们完整的成像数据集可供下载，可用于骨骼生物学，血液学，血管生物学，神经科学和细胞外基质生物学的研究。