We introduce Scallop, an accurate reference-based transcript assembler that improves reconstruction of multi-exon and lowly expressed transcripts. Scallop preserves long-range phasing paths extracted from reads, while producing a parsimonious set of transcripts and minimizing coverage deviation. On 10 human RNA-seq samples, Scallop produces 34.5% and 36.3% more correct multi-exon transcripts than StringTie and TransComb, and respectively identifies 67.5% and 52.3% more lowly expressed transcripts. Scallop achieves higher sensitivity and precision than previous approaches over a wide range of coverage thresholds.

中文翻译：

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通过保存相位的图分解来准确地组装成绩单。

我们介绍了Scallop，这是一种精确的基于参考的转录本汇编程序，可改善多外显子和低表达转录本的重建。扇贝保留了从读取中提取的长距离移相路径，同时生成了一组简化的笔录并最大程度地减少了覆盖范围的偏差。在10个人类RNA-seq样品上，扇贝比StringTie和TransComb产生的正确的多外显子转录子多34.5％和36.3％，分别鉴定出低表达的转录子多67.5％和52.3％。在广泛的覆盖范围阈值范围内，扇贝比以前的方法具有更高的灵敏度和精度。