Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction,Green Chemistry

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Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction
Green Chemistry ( IF 9.3 ) Pub Date : 2017-10-24 00:00:00 , DOI: 10.1039/c7gc02343j
Matthew Wilding _{1,

2,

3,

4} , Thomas S. Peat _{4,

5,

6} , Subha Kalyaanamoorthy _{1,

2,

3,

4} , Janet Newman _{4,

5,

6} , Colin Scott _{1,

2,

3,

4} , Lars S. Jermiin _{1,

2,

3,

4,

7}

Affiliation

The development of new biocatalysts using ancestral sequence reconstruction is reported. When applied to an ω-transaminase, the ancestral proteins demonstrated novel and superior activities with eighty percent of the forty compounds tested compared to the modern day protein, and improvements in activity of up to twenty fold. These included a range of compounds pertinent as feedstocks in polyamide manufacture.

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