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Bulk double emulsification for flow cytometric analysis of microfluidic droplets.
Analyst ( IF 3.6 ) Pub Date : 2017-12-04 , DOI: 10.1039/c7an01695f
David J Sukovich 1 , Samuel C Kim , Noorsher Ahmed , Adam R Abate
Affiliation  

Droplet microfluidics is valuable for applications in chemistry and biology, but generates massive numbers of droplets that must be analyzed and sorted. Here, we describe a simple approach to bulk double emulsify microfluidic emulsions for analysis and sorting with commercial flow cytometers. We illustrate the method by using it to identify droplets based on nucleic acid content. Though simple, our method provides a general approach for analyzing and sorting microfluidic droplets without custom microfluidic double emulsifiers or sorters.

中文翻译:

用于微流体液滴流式细胞术分析的批量双重乳化。

液滴微流体对于化学和生物学应用很有价值,但会产生大量必须进行分析和分类的液滴。在这里,我们描述了一种使用商业流式细胞仪进行分析和分类的批量双乳化微流体乳液的简单方法。我们通过使用它来根据核酸含量识别液滴来说明该方法。虽然简单,但我们的方法提供了一种分析和分类微流体液滴的通用方法,无需定制微流体双乳化器或分类器。
更新日期:2017-11-13
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