The aim of this work was to develop a fast, cost-effective and time-saving liquid chromatography-tandem mass spectrometry (LC–MS/MS) analytical method for the analysis of ethylene glycol (EG) in human serum. For these purposes, the formation/fragmentation of an EG adduct ion with sodium and sodium acetate was applied in the positive electrospray mode for signal detection. Adduct identification was performed with appropriate infusion experiments based on analyte solutions prepared in different concentrations. Corresponding analyte adduct ions and adduct ion fragments could be identified both for EG and the deuterated internal standard (EG-D4). Protein precipitation was used as sample preparation. The analysis of the supernatant was performed with a Luna 5 μm C18 (2) 100 A, 150 mm × 2 mm analytical column and a mobile phase consisting of 95% A (H₂O/methanol = 95/5, v/v) and 5% B (H₂O/methanol = 3/97, v/v), both with 10 mmol L⁻¹ ammonium acetate and 0.1% acetic acid. Method linearity was examined in the range of 100–4000 μg/mL and the calculated limit of detection/quantification was 35/98 μg/mL. However, on the basis of the signal to noise ratio, quantification was recommended at a limit of 300 μg/mL. Additionally, the examined precision, accuracy, stability, selectivity and matrix effect demonstrated that the method is a practicable alternative for EG quantification in human serum. In comparison to other methods based on liquid chromatography, the strategy presented made for the first time the EG analysis without analyte derivatisation possible.

这项工作的目的是开发一种快速，经济高效且省时的液相色谱-串联质谱（LC-MS / MS）分析方法，用于分析人血清中的乙二醇（EG）。为了这些目的，以正电喷雾模式施加与钠和乙酸钠形成/加成EG加合物离子以用于信号检测。基于不同浓度制备的分析物溶液，通过适当的注入实验对加合物进行鉴定。EG和氘代内标（EG-D4）均可鉴定出相应的分析物加合物离子和加合物离子碎片。蛋白沉淀用作样品制备。上清液的分析使用Luna 5μmC18（2）100 A，150 mm×2 mm分析柱和由95％A（H₂ O /甲醇= 95/5，v / v）和5％B（H ₂ O /甲醇= ₃ /97，v / v），均含有10 mmol L ^-1乙酸铵和0.1％乙酸。方法的线性度在100–4000μg/ mL范围内，计算的检测/定量限为35/98μg/ mL。但是，基于信噪比，建议定量限为300μg/ mL。此外，检查的精度，准确性，稳定性，选择性和基质效应证明该方法是人血清中EG定量的可行替代方法。与基于液相色谱的其他方法相比，本文提出的策略首次实现了无需分析物衍生化的EG分析。