Abstract We report a highly luminescent Carbon Dots passivated by nitrogen rich molecules, i.e. N-isopropylacrylamide (NIPAAM), with a QY as high as ca. 94%, which is the highest yield until now. NIPAMM@C-Dots are synthesized directly from the precursors composed of CA, EDA and NIPAAM by hydrothermal reaction. The optical properties of in situ synthesized NIPAAM@C-Dots showed EX-dependent EM at high concentration and EX-independent EM at low concentration. In most cations and anions except Fe 3+ and Bi 3+ added to the aqueous solution, NIPAAM@C-Dots exhibit an extremely stable inert behavior. In addition, in order to verify its usefulness as a fluorescent marker, C6 glioblastoma cells and NIPAAM@C-Dots were cultured together and then applied by both confocal bioimaging and flow cytometry analysis. The role of NIPAAM present on or around the C-Dots surface can improve QY values and stability, resulting in reduced cytotoxicity and can be a strong candidate as an alternative to organic dyes.

中文翻译：

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用于多色生物成像应用的高光致发光 N-异丙基丙烯酰胺 (NIPAAM) 钝化碳点

摘要 我们报告了一种由富氮分子钝化的高发光碳点，即 N-异丙基丙烯酰胺 (NIPAAM)，QY 高达约 94%，这是迄今为止最高的收益率。NIPAMM@C-Dots 由 CA、EDA 和 NIPAAM 组成的前驱体通过水热反应直接合成。原位合成的 NIPAAM@C-Dots 的光学性质在高浓度下显示出依赖于 EX 的 EM，在低浓度下显示出不依赖于 EX 的 EM。除了添加到水溶液中的 Fe 3+ 和 Bi 3+ 外，在大多数阳离子和阴离子中，NIPAAM@C-Dots 表现出极其稳定的惰性行为。此外，为了验证其作为荧光标记物的有效性，将 C6 胶质母细胞瘤细胞和 NIPAAM@C-Dots 一起培养，然后通过共聚焦生物成像和流式细胞术分析应用。