Understanding the mechanisms controlling molecular transport in bioinspired materials is a central topic in many branches of nanotechnology. In this work, we show that biomolecules of fundamental importance in biological processes, such as glucose, can be transported in an active, controlled, and selective manner across macroscopic lipidic cubic mesophases, by correctly reconstituting within them their corresponding membrane protein transporters, such as Staphylococcus epidermidis (GlcP_Se). Importantly, by duly exploiting the symporter properties of GlcP_Se of coupled glucose/H⁺ transport, the diffusion of glucose can further be tuned by independent physiological stimuli, such as parallel or antiparallel pH gradients, offering an important model to study molecular exchange processes in cellular machinery. We finally show that by measuring the transport properties of the lipidic mesophases with and without the GlcP_Se membrane protein reconstituted within, it becomes possible to determine its intrinsic conductance. We generalize these findings to other membrane proteins from the antiporters family, such as the bacterial ClC exchanger from Escherichia coli (EcClC), providing a robust method for evaluating the turnover rate of the membrane proteins in general.

中文翻译：

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活性门控，分子泵送和营业额测定中仿生脂质立方中间相与重构的膜蛋白。

理解控制生物启发材料中分子运输的机制是纳米技术许多分支的中心课题。在这项工作中，我们表明，在生物过程中最重要的生物分子（例如葡萄糖）可以通过在它们之间正确地重构其相应的膜蛋白转运蛋白（例如，葡萄糖）以宏观的脂质立方中间相以主动，受控和选择性的方式进行运输。表皮葡萄球菌（GlcP _Se）。重要的是，通过充分利用偶联的葡萄糖/ H ⁺的GlcP _Se的转运蛋白特性在运输过程中，葡萄糖的扩散可通过独立的生理刺激（例如平行或反平行的pH梯度）进一步调节，从而为研究细胞机械中的分子交换过程提供了重要的模型。我们最终表明，通过测量脂质中间相的运输特性，其中可以在其中重构和不重构GlcP _Se膜蛋白的情况下，确定其固有电导率。我们将这些发现推广到反转运蛋白家族的其他膜蛋白上，例如大肠杆菌（EcClC）的细菌ClC交换子，为评估膜蛋白的总转化率提供了一种可靠的方法。