A bioassay-guided fractionation and chemical investigation of Amaryllis belladonna Steud. bulbs resulted in the isolation and identification of the new crinane alkaloid 1,4-dihydroxy-3-methoxy powellan (1), along with the 3 known crinane alkaloids 2–4 and the two lycorane alkaloids 5–6. The structures were elucidated by interpretation of combined HR-ESIMS, CD and 2D NMR spectroscopic data. Among these isolated compounds the lycorane-type alkaloid acetylcaranine (5) exhibited strong antiplasmodial activity, while compounds 3 and 4 were moderately active, and compounds 1 and 6 were inactive.

中文翻译：

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来自孤挺花颠茄球茎的抗疟原虫生物碱。

孤挺花颠茄的生物测定引导分馏和化学研究。球茎的分离和鉴定导致了新的白鹤烷生物碱 1,4-二羟基-3-甲氧基 Powellan ( 1 )，以及 3 种已知的白鹤烷生物碱2 – 4和两种石蒜烷生物碱5 – 6。通过结合 HR-ESIMS、CD 和 2D NMR 光谱数据的解释来阐明结构。在这些分离的化合物中，石蒜烷型生物碱乙酰卡拉宁( 5 )表现出很强的抗疟原虫活性，而化合物3和4具有中等活性，而化合物1和6则无活性。