Reversible Self-Assembled Monolayers (rSAMs): Adaptable Surfaces for Enhanced Multivalent Interactions and Ultrasensitive Virus Detection.,ACS Central Science

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Reversible Self-Assembled Monolayers (rSAMs): Adaptable Surfaces for Enhanced Multivalent Interactions and Ultrasensitive Virus Detection.
ACS Central Science ( IF 12.7 ) Pub Date : 2017-11-10 , DOI: 10.1021/acscentsci.7b00412
Sing Yee Yeung ₁ , Annabell Mucha ₂ , Ravindra Deshmukh ₂ , Malak Boutrus ₂ , Thomas Arnebrant ₁ , Börje Sellergren _{1,

2}

Affiliation

We report on the design of pH-switchable monolayers allowing a reversible and ordered introduction of affinity reagents on sensor surfaces. The principal layer building blocks consist of α-(4-amidinophenoxy)alkanes decorated at the ω-position with affinity ligands. These spontaneously self-assemble on top of carboxylic acid terminated SAMs to form reversible homo or mixed monolayers (rSAMs) that are tunable with respect to the nature of the head group, layer order and stability while featuring pH responsiveness and the dynamic nature of noncovalent build assemblies. We show that this results in a range of unique biosensor features. As a first example a sialic acid rSAM featuring strong lectin affinity is here used to sense hemagglutinin and influenza virus (H5N1) at the pM and fM level by in situ ellipsometry in a fully reversible fashion. We believe that the rSAM concept will find widespread use in surface chemistry and overall for boosting sensitivity in affinity biosensors.

中文翻译：

可逆自组装单层 (rSAM)：用于增强多价相互作用和超灵敏病毒检测的适应性表面。

我们报告了 pH 可切换单层的设计，允许在传感器表面上可逆且有序地引入亲和试剂。主层结构单元由在 ω 位用亲和配体修饰的 α-(4-脒基苯氧基)烷烃组成。这些自发地在羧酸封端的 SAM 顶部自组装，形成可逆的均质或混合单层 (rSAM)，这些单层在头基性质、层顺序和稳定性方面是可调的，同时具有 pH 响应性和非共价构建的动态性质组件。我们证明这会产生一系列独特的生物传感器特征。作为第一个例子，具有强凝集素亲和力的唾液酸 rSAM 用于通过原位椭圆光度法以完全可逆的方式在 pM 和 fM 水平上感测血凝素和流感病毒 (H5N1)。我们相信 rSAM 概念将在表面化学领域得到广泛应用，并总体上提高亲和生物传感器的灵敏度。

更新日期：2017-11-11

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