Dynamic Control of Aptamer–Ligand Activity Using Strand Displacement Reactions,ACS Synthetic Biology

当前位置： X-MOL 学术 › ACS Synth. Biol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Dynamic Control of Aptamer–Ligand Activity Using Strand Displacement Reactions
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2017-11-10 00:00:00 , DOI: 10.1021/acssynbio.7b00277
Jonathan Lloyd ₁ , Claire H. Tran ₁ , Krishen Wadhwani ₁ , Christian Cuba Samaniego ₂ , Hari K. K. Subramanian ₂ , Elisa Franco ₂

Affiliation

Nucleic acid aptamers are an expandable toolkit of sensors and regulators. To employ aptamer regulators within nonequilibrium molecular networks, the aptamer–ligand interactions should be tunable over time, so that functions within a given system can be activated or suppressed on demand. This is accomplished through complementary sequences to aptamers, which achieve programmable aptamer–ligand dissociation by displacing the aptamer from the ligand. We demonstrate the effectiveness of our simple approach on light-up aptamers as well as on aptamers inhibiting viral RNA polymerases, dynamically controlling the functionality of the aptamer–ligand complex. Mathematical models allow us to obtain estimates for the aptamer displacement kinetics. Our results suggest that aptamers, paired with their complement, could be used to build dynamic nucleic acid networks with direct control over a variety of aptamer-controllable enzymes and their downstream pathways.

中文翻译：

利用链置换反应动态控制适体-配体的活性

核酸适体是传感器和调节剂的可扩展工具包。为了在非平衡分子网络中使用适体调节剂，适体-配体相互作用应随时间调整，以便可以根据需要激活或抑制给定系统内的功能。这是通过与适体的互补序列完成的，该序列通过从配体中置换适体来实现可编程的适体-配体解离。我们证明了我们的简单方法对发光适体以及抑制病毒RNA聚合酶，动态控制适体-配体复合物功能的适体的有效性。数学模型使我们能够获得适体位移动力学的估计。我们的结果表明，适体与其补体配对，

更新日期：2017-11-11

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11