A biosensor for mouse Immunoglobulin G (IgG) was generated from responsive polymer-based interference filters (etalons). To accomplish this, an excess amount of alkaline phosphatase-modified goat anti-mouse IgG (AP-GAM, F(ab')2 fragment specific to mouse IgG) was added to mouse IgG, and allowed to react for some time. After a given reaction time, the bound AP-GAM could be isolated from the unbound, excess AP-GAM by addition of goat anti-mouse IgG (Fc fragment specific)-modified magnetic microspheres (GAM-M) that bind the mouse IgG bound to AP-GAM. After application of a magnetic field, the free, unbound AP-GAM was isolated from the mixture and exposed to an etalon that has its upper Au surface modified with phosphate-containing polymer that can be degraded by AP-GAM. By the phosphate-containing polymer being degraded by the excess AP-GAM, the cleaved phosphate groups can diffuse into the interference filter's active polymer layer that yields a change in the optical properties that can be related to the amount of IgG in the sample. This concept is extremely straightforward to implement, and can be modified to detect a variety of other analytes of interest.

中文翻译：

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基于聚（N-异丙基丙烯酰胺）光学器件的酶辅助聚合物膜降解生物分子传感

小鼠免疫球蛋白 G (IgG) 的生物传感器是从基于响应聚合物的干涉滤光片（标准具）中产生的。为此，将过量的碱性磷酸酶修饰的山羊抗小鼠 IgG（AP-GAM，小鼠 IgG 特异性的 F(ab')2 片段）添加到小鼠 IgG 中，并使其反应一段时间。在给定的反应时间后，可以通过添加与小鼠 IgG 结合的山羊抗小鼠 IgG（Fc 片段特异性）修饰的磁性微球 (GAM-M) 将结合的 AP-GAM 与未结合的过量 AP-GAM 分离到 AP-GAM。施加磁场后，游离的、未结合的 AP-GAM 从混合物中分离出来，并暴露于标准具，该标准具的上金表面用含磷酸盐的聚合物改性，可被 AP-GAM 降解。由于含磷酸盐的聚合物被过量的 AP-GAM 降解，裂解的磷酸基团可以扩散到干涉滤光片的活性聚合物层中，从而导致光学特性发生变化，这可能与样品中 IgG 的含量有关。这个概念实施起来非常简单，可以修改以检测各种其他感兴趣的分析物。