Recent advancements in separation science have resulted in the commercialization of multidimensional separation systems that provide higher peak capacities and, hence, enable a more-detailed characterization of complex mixtures. In particular, two powerful analytical tools are increasingly used by analytical scientists, namely online comprehensive two-dimensional liquid chromatography (LC × LC, having a second-dimension separation in the liquid phase) and liquid chromatography-ion mobility-spectrometry (LC-IMS, second dimension separation in the gas phase).

The goal of the current study was a general assessment of the liquid-chromatography–trapped-ion-mobility–mass spectrometry (LC-TIMS–MS) and comprehensive two-dimensional liquid chromatography–mass spectrometry (LC × LC–MS) platforms for untargeted lipid mapping in human plasma.

For the first time trapped-ion-mobility spectrometry (TIMS) was employed for the separation of the major lipid classes and ion-mobility-derived collision-cross-section values were determined for a number of lipid standards. The general effects of a number of influencing parameters have been inspected and possible directions for improvements are discussed.

We aimed to provide a general indication and practical guidelines for the analyst to choose an efficient multidimensional separation platform according to the particular requirements of the application. Analysis time, orthogonality, peak capacity, and an indicative measure for the resolving power are discussed as main characteristics for multidimensional separation systems.

分离科学的最新进展已导致多维分离系统的商业化，该系统可提供更高的峰容量，因此可对复杂混合物进行更详细的表征。特别地，分析科学家越来越多地使用两种强大的分析工具，即在线全面二维液相色谱（LC×LC，在液相中具有第二维分离）和液相色谱-离子迁移谱法（LC-IMS）。 ，气相中的二维分离）。

本研究的目的是对液相色谱-捕获离子迁移-质谱（LC-TIMS-MS）和全面的二维液相色谱-质谱（LC×LC-MS）平台进行总体评估。人血浆中的非靶向脂质作图。

首次采用了离子淌度质谱（TIMS）分离主要脂质类别，并确定了许多脂质标准品的离子淌度碰撞截面值。已经检查了许多影响参数的一般效果，并讨论了可能的改进方向。

我们旨在为分析人员根据应用程序的特定需求选择有效的多维分离平台提供一般指示和实用指南。分析时间，正交性，峰容量和分辨能力的指示性度量作为多维分离系统的主要特征进行了讨论。