The interest in DNA–protein-based diagnostics has recently been growing enormously, which makes the separation process of DNA or protein from a cell extract extremely important. Unlike the traditional separation process, a novel approach is in demand which can nondestructively isolate the target biomolecules without sacrificing the other components in the mixture. In this study, we have demonstrated a new and simple separation technique by using well-established bifunctional Au–Fe₃O₄ nanocomposites as the separation nanoprobes to efficiently isolate the specifically targeted nanomolar concentrated DNA over 70% from its associate DNA–protein mixture in the presence of a magnetic field. The sensing accuracy of both as-separated DNA and protein are quantitatively examined by UV–vis spectroscopy, and then qualitatively validated by gel analysis. Results obtained in this study clearly demonstrated that this newly developed separation procedure not only provides the efficient separation for the targeted DNA but can also maintain the bioactivity of as-separated protein and DNA solutions. The superiority of this technique can open an avenue to establish a label-free and nondestructive platform for a wide variety of biomolecule separation applications.

中文翻译：

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使用磁性Au–Fe ₃ O ₄纳米探针从DNA蛋白质混合物中分离目标DNA的无标记无损分离技术

近来，人们对基于DNA蛋白质的诊断方法的兴趣日益增长，这使得从细胞提取物中分离DNA或蛋白质的过程极为重要。与传统的分离方法不同，需要一种新颖的方法，该方法可以无损地分离目标生物分子，而无需牺牲混合物中的其他组分。在这项研究中，我们通过使用公认的双功能Au–Fe ₃ O ₄展示了一种新的简单分离技术纳米复合物作为分离纳米探针，可以在存在磁场的情况下从其相关的DNA-蛋白质混合物中有效地分离超过70％的特定目标的纳摩尔浓缩DNA。分离的DNA和蛋白质的感测准确性通过UV-vis光谱法进行定量检查，然后通过凝胶分析进行定性验证。这项研究获得的结果清楚地表明，这种新开发的分离程序不仅可以为目标DNA提供有效的分离，而且还可以保持分离后的蛋白质和DNA溶液的生物活性。该技术的优越性可以为建立用于多种生物分子分离应用的无标签，无损平台提供途径。