Here, a nontoxic antibacterial material based on Chitosan Oligosaccharide-N-Chlorokojic acid Mannich base (COS-N-MB) that was synthesized by using the selective partial alkylation reaction displaying excellent activity against bacterial infection. The proposed mechanism of the action of COS-N-MB is that this antibacterial material with positive charge and synergistic antibacterial effects can promote it’s adsorption to bacterial cell wall through electrostatic interaction and chelating metal cations. It changed the permeability of the membrane, caused cellular leakage, and destroyed the membrane integrity, leading to complete membrane disruption and eventually death of the bacteria. Besides, COS-N-MB can interact with membrane proteins, causing deformation in the structure and functionality. The good biocompatibility, noncytotoxic, and low hemolysis made this novel material a promising and effective compound for antibacterial applications.

在此，通过使用选择性部分烷基化反应合成的基于壳聚糖寡糖-N-氯代果酸曼尼希碱（COS-N-MB）的无毒抗菌材料显示出优异的抗细菌感染活性。提出的COS-N-MB作用机理是这种具有正电荷和协同抗菌作用的抗菌材料可以通过静电相互作用和螯合金属阳离子促进其吸附到细菌细胞壁上。它改变了膜的渗透性，引起细胞渗漏，破坏了膜的完整性，导致膜完全破坏，最终导致细菌死亡。此外，COS-N-MB可以与膜蛋白相互作用，从而引起结构和功能的变形。良好的生物相容性，无细胞毒性，