Carbohydrate Polymers ( IF 10.7 ) Pub Date : 2017-11-03 , DOI: 10.1016/j.carbpol.2017.11.016 Mamoni Dash , Sangram K. Samal , Andrea Morelli , Cristina Bartoli , Heidi A. Declercq , Timothy E.L. Douglas , Peter Dubruel , Federica Chiellini
Polyelectrolyte complexes (PEC) of chitosan and ulvan were fabricated to study alkaline phosphatase (ALP) mediated formation of apatitic minerals. Scaffolds of the PEC were subjected to ALP and successful mineral formation was studied using SEM, Raman and XRD techniques. Investigation of the morphology via SEM shows globular structures of the deposited minerals, which promoted cell attachment, proliferation and extracellular matrix formation. The PEC and their successful calcium phosphate based mineralization offers a greener route of scaffold fabrication towards developing resorbable materials for tissue engineering.
中文翻译:
Ulvan-壳聚糖聚电解质复合物作为酶诱导仿生矿化的基质
制备了壳聚糖和ulvan的聚电解质复合物(PEC),以研究碱性磷酸酶(ALP)介导的脂肪性矿物质的形成。对PEC的脚手架进行ALP,并使用SEM,拉曼和XRD技术研究了成功的矿物形成。通过SEM对形态的研究显示出沉积矿物质的球状结构,其促进了细胞附着,增殖和细胞外基质的形成。PEC及其成功的基于磷酸钙的矿化方法为开发用于组织工程的可吸收材料提供了一条更绿色的脚手架制造路线。